{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:16:35Z","timestamp":1753881395871,"version":"3.41.2"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T00:00:00Z","timestamp":1565136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T00:00:00Z","timestamp":1566950400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01 GM110227","R01 GM113890"],"award-info":[{"award-number":["R01 GM110227","R01 GM113890"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["F30 GM125117"],"award-info":[{"award-number":["F30 GM125117"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,8,28]]},"abstract":"<jats:p>\n            <jats:named-content content-type=\"genus-species\">Trypanosoma brucei<\/jats:named-content>\n            is a parasite responsible for human and animal African trypanosomiasis. Current treatments for these diseases have numerous problems, and the development of novel chemotherapeutics can be achieved by identifying targets that are parasite specific and part of essential processes. Ribosome biogenesis is the process of generating translation-competent ribosomes and is critical for survival in all organisms. Work from our laboratory has shown that the formation of the 5S RNP, a crucial checkpoint in ribosome biogenesis, requires trypanosome-specific proteins P34\/P37 and homologues of Rpf2 and L5 which possess parasite-specific characteristics. In this study, we characterize TbRrs1, an additional member of the\n            <jats:named-content content-type=\"genus-species\">T. brucei<\/jats:named-content>\n            5S RNP, and show that it is essential for parasite survival and has unique interactions with P34\/P37 and 5S rRNA. This expands our understanding of the 5S RNP in\n            <jats:named-content content-type=\"genus-species\">T. brucei<\/jats:named-content>\n            and identifies new targets for future drug development.\n          <\/jats:p>","DOI":"10.1128\/msphere.00453-19","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T13:23:24Z","timestamp":1565097804000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Trypanosoma brucei Homologue of Regulator of Ribosome Synthesis 1 (Rrs1) Has Direct Interactions with Essential Trypanosome-Specific Proteins"],"prefix":"10.1128","volume":"4","author":[{"given":"Daniel","family":"Jaremko","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York, 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