{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:18:28Z","timestamp":1790745508246,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Elderly Health Project of Sichuan Provincial Health Commission","award":["GBKT20021"],"award-info":[{"award-number":["GBKT20021"]}]},{"name":"SPDST | Natural Science Foundation of Sichuan Province","award":["2023NSFSC1467"],"award-info":[{"award-number":["2023NSFSC1467"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2023,12,20]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Gut microbiota plays a crucial role in providing colonization resistance against multi-drug resistant organisms including carbapenem-resistant\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            (CRKP). However, the impact of different carbapenems on intestinal colonization resistance against CRKP remains poorly understood. In this study, we aimed to investigate the effects of three commonly used carbapenems (meropenem, imipenem, and ertapenem) administered at single-day doses, which are typically employed in emergency departments for managing infected patients, on CRKP gut colonization. We conducted experiments in mice by intravenously administering each carbapenem using human-simulated one-day regimens. The composition of the gut microbiota was analyzed using 16S rRNA amplicon sequencing before and after carbapenem administration. Our results revealed that all three carbapenems, when administered at a single-day dose significantly altered the abundance and diversity of the gut microbiota, leading to compromised colonization resistance against CRKP. Notably, the ertapenem and imipenem groups showed an increase in\n            <jats:italic>Allobaculum<\/jats:italic>\n            ,\n            <jats:italic>Bifidobacterium<\/jats:italic>\n            ,\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            , while the meropenem and imipenem groups exhibited a decrease in\n            <jats:italic>Ruminococcaceae<\/jats:italic>\n            , S24-7, and\n            <jats:italic>Akkermania<\/jats:italic>\n            . Additionally, the Shannon index exhibited a negative correlation with both the number of days of CRKP colonization CFUs and the duration of bacteria shedding. Furthermore, the count of CRKP in mice after ertapenem administration on the first day and the duration of CRKP shedding were significantly lower compared to meropenem and imipenem. Predictive metabolic pathway analysis demonstrated that the three carbapenems similarly affected a range of metabolic pathways of gut microflora, including carbohydrate metabolism and vitamin B. Our findings emphasize that ertapenem, with its relatively narrow spectrum, minimizes perturbations of the gut microbiota and has a relatively less impact on gut colonization resistance against CRKP.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The intestinal colonization of carbapenem-resistant\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              (CRKP) is an important source of clinical infection. Our research showed that even single-day dose use of carbapenems caused CRKP colonization and continuous bacterial shedding, which reminds clinical doctors to prescribe carbapenems cautiously. Whenever possible, ertapenem should be the preferred choice over other carbapenems especially when the identified or highly suspected pathogens can be effectively targeted by ertapenem.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00479-23","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T14:05:16Z","timestamp":1701093916000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["The impact of three carbapenems at a single-day dose on intestinal colonization resistance against carbapenem-resistant\n            <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1128","volume":"8","author":[{"given":"Huan","family":"Kuang","sequence":"first","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Luo","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China"},{"name":"Center for Pathogen Research, West China Hospital, Sichuan University, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3650-7298","authenticated-orcid":false,"given":"Xiaoju","family":"Lv","sequence":"additional","affiliation":[{"name":"Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China"},{"name":"Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12020234"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-022-03943-z"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz622"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3535"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12563"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00891-10"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.11.3610-3612.2003"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.03.022"},{"key":"e_1_3_5_10_2","first-page":"9","article-title":"Management measures for clinical application of Antibacterial drugs","volume":"5","author":"Anonymous","year":"2013","unstructured":"Anonymous. 2013. Management measures for clinical application of Antibacterial drugs. Chin J of the Front of Med Sci (Electronic Version) 5:9\u201314.","journal-title":"Chin J of the Front of Med Sci (Electronic Version)"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.03.023"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2602.190594"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0548-6"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/00498250410001670643"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.3109\/00498259309059441"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(85)90101-9"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.202270001"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.230014"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.07.002"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.10.043"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-022-00650-6"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-020-1289-4"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2022.05.004"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.09.004"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00194-07"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00933-y"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.02.002"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-1356-0"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3217-3221.2005"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00833-7"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu12113380"},{"key":"e_1_3_5_33_2","first-page":"283","article-title":"Crosstalk between vitamin B and immunity","volume":"29","author":"Spinas E","year":"2015","unstructured":"Spinas E, Saggini A, Kritas SK, Cerulli G, Caraffa A, Antinolfi P, Pantalone A, Frydas A, Tei M, Speziali A, Saggini R, Pandolfi F, Conti P. 2015. Crosstalk between vitamin B and immunity. J Biol Regul Homeost Agents 29:283\u2013288.","journal-title":"J Biol Regul Homeost Agents"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343940"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu5124836"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.04.017"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2016.09.008"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135515"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.120725"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.10.489"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.06.007"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/8.19.4321"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000080107"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0209-9"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.139"},{"key":"e_1_3_5_47_2","unstructured":"FGB JO Kindt R Legendre P Minchin PR O\u2019Hara RB Simpson GL Peter Solymos MHHS Wagner H. 2022. Ggplot2: elegant graphics for data analysis"},{"key":"e_1_3_5_48_2","doi-asserted-by":"crossref","unstructured":"Wickham H. 2016. Ggplot2: Elegant Graphics for data analysis","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baaa055"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.16288\/j.yczz.20-080"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00479-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00479-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T14:06:40Z","timestamp":1703081200000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00479-23"}},"subtitle":[],"editor":[{"given":"Patricia A.","family":"Bradford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,12,20]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,12,20]]}},"alternative-id":["10.1128\/msphere.00479-23"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00479-23","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,20]]},"assertion":[{"value":"2023-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00479-23"}}