{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:52:29Z","timestamp":1791942749031,"version":"4.3.4"},"reference-count":75,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Guangdong Medical Science and Technology Research Fund Project","award":["B2023232"],"award-info":[{"award-number":["B2023232"]}]},{"name":"President Foundation of The Fifth Affiliated Hospital, Southern Medical University","award":["YZ2022ZX01"],"award-info":[{"award-number":["YZ2022ZX01"]}]},{"name":"Guangdong Basic and Applied Basic Research Fund Provincial Enterprise Joint Fund","award":["2021A1515220153"],"award-info":[{"award-number":["2021A1515220153"]}]},{"name":"Military Logistics Research Fund Project","award":["CLB21J018"],"award-info":[{"award-number":["CLB21J018"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2023,12,20]]},"abstract":"ABSTRACT<\/jats:title>\n \n The detection rate of carbapenem-resistant nontyphoidal\n Salmonella<\/jats:italic>\n (NTS) is relatively low. However, carbapenem-sensitive and carbapenem-resistant\n Salmonella enterica<\/jats:italic>\n serovar Typhimurium (\n S. enterica<\/jats:italic>\n serovar Typhimurium) were isolated from a clinical outpatient within a span of 10 days, named 1104\u201365 and 1104\u201375. We aimed to reveal the mechanism of carbapenem resistance in\n S. enterica<\/jats:italic>\n serovar Typhimurium isolates in this case. The resistance phenotype of\n S. enterica<\/jats:italic>\n serovar Typhimurium was analyzed by the VITEK-2 Compact equipment and Kirby-Bauer disc diffusion method. Whole-genome sequencing was used to analyze the serotype, ST type, genetic relationship, resistance genes, plasmid replicon, the genetic environment of\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n , and the characteristics of IncFII plasmid carrying\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n in\n S. enterica<\/jats:italic>\n serovar Typhimurium. The transferability of the IncFII plasmid carrying\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n was verified by the conjugation assay and PCR. The serotypes of both NTS are\n S. enterica<\/jats:italic>\n serovar Typhimurium, belonging to ST34. Single nucleotide polymorphisms analysis showed that they were the same clone. A conjugative IncFII plasmid pIncFII-NDM5 with transferability was detected in isolate 1104\u201375, which harboring the\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n gene was the primary mechanism responsible for mediating carbapenem resistance in\n S. enterica<\/jats:italic>\n serovar Typhimurium. The genetic environment of\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n on pIncFII-NDM5 is \u201cIS\n 26<\/jats:italic>\n -\u0394IS\n 3000<\/jats:italic>\n -IS\n 5<\/jats:italic>\n -\u0394IS\n Aba125-bla<\/jats:italic>\n NDM-5<\/jats:sub>\n -\n ble<\/jats:italic>\n MBL<\/jats:sub>\n -\n trpF-dsbC -cutA<\/jats:italic>\n -IS\n 26<\/jats:italic>\n \u201d, which was confirmed as a novel structure not previously reported in the NCBI database. Although the mechanism is rarely reported in NTS, the prevalence of IncFII plasmid pIncFII-NDM5 will pose a great threat to the clinical treatment of\n S. enterica<\/jats:italic>\n serovar Typhimurium. Meanwhile, the coexistence of\n bla<\/jats:italic>\n CTX-M-55<\/jats:sub>\n ,\n qnrS1<\/jats:italic>\n ,\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n , and\n mph(A<\/jats:italic>\n ) in\n S. enterica<\/jats:italic>\n serovar Typhimurium ST34 warrants additional attention.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n In this study, an IncFII plasmid pIncFII-NDM5 carrying\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n was found in carbapenem-resistant\n Salmonella enterica<\/jats:italic>\n serovar Typhimurium (\n S. enterica<\/jats:italic>\n serovar Typhimurium), which has conjugative transferability and carried\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n ,\n ble<\/jats:italic>\n MBL<\/jats:sub>\n ,\n mph(A<\/jats:italic>\n ), and\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n four resistance genes that can mediate resistance to multiple antibiotics including cephalosporins, beta-lactamase inhibitor combinations, carbapenems, and macrolides. Phylogenetic analysis showed that 1104\u201365 and 1104\u201375 were closely related to other\n S. enterica<\/jats:italic>\n serovar Typhimurium in this area. The above-mentioned\n S. enterica<\/jats:italic>\n serovar Typhimurium chromosome carries\n bla<\/jats:italic>\n CTX-M-55<\/jats:sub>\n ,\n qnrS1<\/jats:italic>\n , and\n tet(A<\/jats:italic>\n ) genes, so the antibiotic resistance of isolates will be further enhanced after obtaining the pIncFII_NDM5-like plasmid. Meanwhile, we discovered a novel genetic structure of\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n mediated by the IS\n 26<\/jats:italic>\n composite transposon, which will expand our understanding of the emergence and spread of carbapenem-resistance genes. Altogether, the presence of the IncFII plasmid pIncFII-NDM5 further underscores the need for vigilant surveillance and appropriate infection control measures to mitigate the impact of carbapenem-resistant\n S. enterica<\/jats:italic>\n serovar Typhimurium in clinical settings.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/msphere.00480-23","type":"journal-article","created":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T09:42:09Z","timestamp":1698831729000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Molecular characterization of IncFII plasmid carrying\n bla<\/i>\n NDM-5<\/sub>\n in a\n Salmonella enterica<\/i>\n serovar Typhimurium ST34 clinical isolate in China"],"prefix":"10.1128","volume":"8","author":[{"given":"Shihan","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Fifth Affiliated Hospital, Southern Medical University, Guangzhou, 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