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However, the ST95 lineage is defined by low levels of drug resistance amongst clinical isolates, which normally provides for uncomplicated treatment options. Here, we provide the first detailed genomic analysis of an\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    ST95 isolate that has both high virulence potential and resistance to multiple antibiotics. Using the genome, we predicted its virulence and antibiotic resistance mechanisms, which include resistance to last-line antibiotics mediated by the plasmid-borne\n                    <jats:italic>mcr-1<\/jats:italic>\n                    gene. Finding an ST95 isolate resistant to nearly all antibiotics that also has a high virulence potential is of major clinical importance and underscores the need to monitor new and emerging trends in antibiotic resistance development in this important global lineage.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00486-18","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T04:39:29Z","timestamp":1539059969000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Discovery of\n                    <i>mcr-1<\/i>\n                    -Mediated Colistin Resistance in a Highly Virulent Escherichia coli Lineage"],"prefix":"10.1128","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2264-4785","authenticated-orcid":false,"given":"Brian M.","family":"Forde","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia"},{"name":"Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia"},{"name":"Australian Centre for Ecogenomics, The University of Queensland, Brisbane, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9718-6475","authenticated-orcid":false,"given":"Hosam M.","family":"Zowawi","sequence":"additional","affiliation":[{"name":"Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia"},{"name":"The University of Queensland, UQ Centre for Clinical Research (UQCCR), Herston, Queensland, Australia"},{"name":"College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia"},{"name":"World Health Organization Collaborating Centre for Infection Prevention and Control, Riyadh, Saudi Arabia"},{"name":"Gulf Cooperation Council Center for Infection Control, Riyadh, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2895-0345","authenticated-orcid":false,"given":"Patrick N. 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