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If host immunity wanes, cysts reactivate to cause severe, and often lethal, toxoplasmic encephalitis. There is no available therapy to eliminate cysts or to prevent their reactivation. Moreover, how the vital and characteristic cyst matrix and cyst wall structures develop is poorly understood. Here, we visualized and tracked the localization of\n            <jats:italic>Toxoplasma<\/jats:italic>\n            intravacuolar-network-associated dense granule (GRA) proteins during cyst development\n            <jats:italic>in vitro<\/jats:italic>\n            . Intravacuolar-network GRAs were present within the cyst matrix and at the cyst wall in developing cysts, and genetic deletion of intravacuolar-network-associated GRAs reduced the rate of accumulation of cyst wall material at the cyst periphery. Our results show that intravacuolar-network-associated GRAs, particularly GRA2 and GRA12, play dynamic and essential roles in the development and maturation of the cyst matrix and the cyst wall structures.\n          <\/jats:p>","DOI":"10.1128\/msphere.00487-19","type":"journal-article","created":{"date-parts":[[2019,10,15]],"date-time":"2019-10-15T11:18:16Z","timestamp":1571138296000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Toxoplasma gondii Intravacuolar-Network-Associated Dense Granule Proteins Regulate Maturation of the Cyst Matrix and Cyst Wall"],"prefix":"10.1128","volume":"4","author":[{"given":"Rebekah B.","family":"Guevara","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara A.","family":"Fox","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Falla","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Bzik","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090203"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.11.024"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0020-7519(00)00124-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.09.005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40124-014-0055-7"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0164-4"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/15.2.211"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.02.004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2013.51.4.393"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094717"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165740"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2017.09.006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.2741\/weiss"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005447"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.4303\/jnp\/N100505"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003823"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/bf00535321"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01681.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.267"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050315"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.3791\/2091"},{"key":"e_1_3_2_24_2","first-page":"18S","article-title":"Development of bradyzoites of Toxoplasma gondii in vitro","volume":"41","author":"Weiss LM","year":"1994","unstructured":"Weiss LM, Laplace D, Takvorian PM, Cali A, Tanowitz HB, Wittner M. 1994. 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