{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:32:00Z","timestamp":1772163120281,"version":"3.50.1"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:00:00Z","timestamp":1676937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:00:00Z","timestamp":1676937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21J23396"],"award-info":[{"award-number":["JP21J23396"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K06413"],"award-info":[{"award-number":["JP20K06413"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2023,2,21]]},"abstract":"<jats:p>While pathogen-level quantification is an important diagnostic of disease severity and transmissibility, disease-related host biomarkers are also useful in predicting outcomes in infectious diseases. In this study, we demonstrate that combined proviral load (PVL) and host biomarker diagnostics can be used to detect bovine leukemia virus (BLV) infection, which has a negative economic impact on the cattle industry.<\/jats:p>","DOI":"10.1128\/msphere.00493-22","type":"journal-article","created":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T10:00:02Z","timestamp":1673344802000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Identifying Pathogen and Allele Type Simultaneously in a Single Well Using Droplet Digital PCR"],"prefix":"10.1128","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9152-471X","authenticated-orcid":true,"given":"Kosuke","family":"Notsu","sequence":"first","affiliation":[{"name":"Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hala","family":"El Daous","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Benha University, Toukh, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuya","family":"Mitoma","sequence":"additional","affiliation":[{"name":"Department of infectious disease, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyue","family":"Wu","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junzo","family":"Norimine","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan"},{"name":"Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2066-128X","authenticated-orcid":true,"given":"Satoshi","family":"Sekiguchi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan"},{"name":"Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19057-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2016.11.008"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.1.88"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e32832b7dca"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69302"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30985-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3084"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00292"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2022.102178"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1207-1"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2013.07.013"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43016-020-0057-2"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2014-9241"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0749-0720(15)30368-6"},{"key":"e_1_3_2_16_2","unstructured":"World Organization of Animal Health. 2022. Terrestrial animal health code. https:\/\/www.woah.org\/en\/what-we-do\/standards\/codes-and-manuals\/terrestrial-code-online-access\/. Accessed 22 July 2022."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-017-0876-4"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1943-4"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00328"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2003.08.003"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2021.105528"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(88)90066-1"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7112929"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14285"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12041"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/age.12566"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani11030647"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2008.01750.x"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12977-019-0476-z"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.16-0601"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac403843j"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2021.100243"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/tan.14502"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.1992.tb00168.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.11606\/issn.1678-4456.bjvras.2017.123769"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.02.024"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(03)00057-6"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-10480"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.14-0624"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018-16186"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-0929.2006.00332.x"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.46440"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-5347(88)90058-4"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-020-03372-8"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10499-021-00747-w"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/f11111190"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.11.009"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16124-9"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00510-20"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.09.011"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-019-0732-1"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-1119(03)00744-3"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2011.02264.x"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11101093"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/tan.12692"}],"updated-by":[{"DOI":"10.1128\/msphere.00208-23","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T00:00:00Z","timestamp":1686614400000}}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00493-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00493-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T09:44:33Z","timestamp":1676972673000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00493-22"}},"subtitle":[],"editor":[{"given":"Matthew B.","family":"Frieman","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,2,21]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2,21]]}},"alternative-id":["10.1128\/msphere.00493-22"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00493-22","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.09.10.507438","asserted-by":"object"}]},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,21]]},"assertion":[{"value":"2022-10-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00493-22"}}