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Widespread resistance to current antimalarials and the lack of a commercial vaccine necessitate novel pharmacological interventions with distinct modes of action against malaria. For toxoplasmosis, new drugs to effectively eliminate tissue-dwelling latent cysts of the parasite are needed. The Malaria Box antimalarial collection, managed and distributed by the Medicines for Malaria Venture, includes molecules of novel chemical classes with proven antimalarial efficacy. Using targeted phenotypic assays of\n            <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            , we have identified a subset of the Malaria Box molecules as potent inhibitors of plastid segregation and parasite invasion and egress, thereby providing early insights into their probable mode of action. Five molecules that inhibit the egress of both parasites have been identified for further mechanistic studies. Thus, the approach we have used to identify novel molecules with defined modes of action in multiple parasites can expedite the development of pan-active antiparasitic agents.\n          <\/jats:p>","DOI":"10.1128\/msphere.00534-17","type":"journal-article","created":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T11:26:02Z","timestamp":1516101962000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Targeted Phenotypic Screening in Plasmodium falciparum and Toxoplasma gondii Reveals Novel Modes of Action of Medicines for Malaria Venture Malaria Box Molecules"],"prefix":"10.1128","volume":"3","author":[{"given":"Gowtham","family":"Subramanian","sequence":"first","affiliation":[{"name":"Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenakshi A.","family":"Belekar","sequence":"additional","affiliation":[{"name":"Biochemical Sciences Division, CSIR National Chemical Laboratory, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anurag","family":"Shukla","sequence":"additional","affiliation":[{"name":"Biochemical Sciences Division, CSIR National Chemical Laboratory, Pune, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie Xin","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, National University of Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7959-075X","authenticated-orcid":false,"given":"Ameya","family":"Sinha","sequence":"additional","affiliation":[{"name":"Pillar of Engineering Product Development, Singapore University of Technology and Design, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trang T. 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