{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:51:38Z","timestamp":1790635898646,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Programme Op\u00e9rationnel FEDER-Guadeloupe-Conseil Regional","award":["\"Une Sant\u00e9\" 2021-2024"],"award-info":[{"award-number":["\"Une Sant\u00e9\" 2021-2024"]}]},{"DOI":"10.13039\/501100023300","name":"Institut Pasteur","doi-asserted-by":"publisher","award":["Calmette & Yersin postdoctoral fellowship"],"award-info":[{"award-number":["Calmette & Yersin postdoctoral fellowship"]}],"id":[{"id":"10.13039\/501100023300","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,12,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Mosquito rearing optimization in laboratory conditions is crucial for both vector research and control. Although the addition of nutrients is important for\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      development from immature stages to adult mosquitoes, little is known about the nutrient composition of commercial diets used for mosquito rearing and their influence on\n                      <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                      life traits. Here, we evaluated the influence of four commercial diets commonly used to rear\n                      <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                      in the laboratory on its fitness, lifespan, and microbiota. We also compared the effect of these diets on this mosquito when combined with two different rearing waters (laboratory versus field-collected waters). Our investigations demonstrated that higher levels of protein and lipid in commercial diets promote better\n                      <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                      development, lifespan, and size in both water. Metagenomic analysis revealed specific modulations of adult microbiota composition according to both diet and rearing water.\n                      <jats:italic toggle=\"yes\">Chryseobacterium<\/jats:italic>\n                      dominated the microbiota of female mosquitoes reared in laboratory water, except for yeast condition, where a more diverse microbiota was observed. When reared in larval site water, the microbiota diversity was overall higher despite diet addition, except for fish food, which promoted\n                      <jats:italic toggle=\"yes\">Sphingobacterium<\/jats:italic>\n                      dominance. Given the pivotal influence of diet addition during the larval stage on\n                      <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                      microbiota and life traits, rearing conditions should be carefully chosen according to the goals of the research (i.e., vectorial capacity estimations) or vector control intervention.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                        is the main vector of arbovirus, such as dengue, yellow fever, and chikungunya viruses. Vector research and control are primarily carried out in laboratories, with larval stage rearing conducted using commercial diet. If many nutrients are essential for\n                        <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                        development, gaining insight into the influence of these diets and their nutrient levels is important to promote optimized rearing worldwide. In this study, our results indicated a significant impact of commercial diet on\n                        <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                        development, lifespan, size, and microbiota related to contrasted protein, lipid, and carbohydrate levels in these diets. This study will help people working with\n                        <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                        raise awareness in staff working with\n                        <jats:italic toggle=\"yes\">Ae. aegypti<\/jats:italic>\n                        to select optimized diets for their specific purpose.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00543-25","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T13:43:36Z","timestamp":1762782216000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Contrasted impacts of commercial diets and rearing water on\n                    <i>Aedes aegypti<\/i>\n                    fitness and microbiota"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5772-8161","authenticated-orcid":true,"given":"Elodie","family":"Calvez","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0495fxg12","id-type":"ROR","asserted-by":"publisher"}],"name":"Vector-Borne Diseases Laboratory, Environment and Health Research Department, Institut Pasteur de la Guadeloupe","place":["Les Abymes, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaure","family":"Qu\u00e9tel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0495fxg12","id-type":"ROR","asserted-by":"publisher"}],"name":"Microbial Ecology team, Environment and Health Research Department, Institut Pasteur de la Guadeloupe","place":["Les Abymes, 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