{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:04:26Z","timestamp":1792677866472,"version":"4.5.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2018,11,7]],"date-time":"2018-11-07T00:00:00Z","timestamp":1541548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T00:00:00Z","timestamp":1545782400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100011912","name":"WUSTL | Washington University School of Medicine in St. Louis","doi-asserted-by":"publisher","award":["Start Up"],"award-info":[{"award-number":["Start Up"]}],"id":[{"id":"10.13039\/100011912","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2018,12,26]]},"abstract":"<jats:p>\n                    Species from the\n                    <jats:italic>Bacteroides<\/jats:italic>\n                    genus are predominant members of the human gut microbiota. OMVs in\n                    <jats:italic>Bacteroides<\/jats:italic>\n                    have been shown to be important for the homeostasis of complex host-commensal relationships, mainly involving immune tolerance and protection from disease. OMVs carry many enzymatic activities involved in the cleavage of complex polysaccharides and have been proposed as public goods that can provide growth to other bacterial species by release of polysaccharide breakdown products into the gut lumen. This work shows that the presence of a negatively charged rich amino acid motif (LES) is required for efficient packing of the surface-exposed alpha-amylase SusG into OMVs. Our findings strongly suggest that surface exposure is coupled to packing of\n                    <jats:italic>Bacteroides<\/jats:italic>\n                    lipoproteins into OMVs. This is the first step in the generation of tailor-made probiotic interventions that can exploit LES-related sequences to generate\n                    <jats:italic>Bacteroides<\/jats:italic>\n                    strains displaying proteins of interest in OMVs.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00559-18","type":"journal-article","created":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T05:48:22Z","timestamp":1541483302000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":117,"title":["Surface Exposure and Packing of Lipoproteins into Outer Membrane Vesicles Are Coupled Processes in\n                    <i>Bacteroides<\/i>"],"prefix":"10.1128","volume":"3","author":[{"given":"Ezequiel","family":"Valguarnera","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology, Washington University in Saint Louis, Saint Louis, Missouri, 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