{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T18:16:48Z","timestamp":1776363408492,"version":"3.51.2"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T00:00:00Z","timestamp":1546387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T00:00:00Z","timestamp":1551225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000774","name":"DOD | Defense Threat Reduction Agency","doi-asserted-by":"publisher","award":["HDTRA-17-C-0015"],"award-info":[{"award-number":["HDTRA-17-C-0015"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,2,27]]},"abstract":"<jats:p>\n            In recent years, an increasing number of melioidosis cases have been reported in several regions where melioidosis is endemic and in areas where melioidosis had not commonly been diagnosed. Currently, the estimated burden of disease is around 165,000 new cases annually, including 89,000 cases that have fatal outcomes. This life-threatening infectious disease is caused by\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            , which is classified as a Tier 1 select agent. Due to the high case fatality rate, intrinsic resistance to multiple antibiotic treatments, susceptibility to infection via the aerosol route, and potential use as a bioweapon, we have developed an effective live attenuated PBK001 vaccine capable of protecting against aerosolized melioidosis.\n          <\/jats:p>","DOI":"10.1128\/msphere.00570-18","type":"journal-article","created":{"date-parts":[[2019,1,3]],"date-time":"2019-01-03T13:12:29Z","timestamp":1546521149000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Burkholderia pseudomallei \u0394\n            <i>tonB<\/i>\n            \u0394\n            <i>hcp1<\/i>\n            Live Attenuated Vaccine Strain Elicits Full Protective Immunity against Aerosolized Melioidosis Infection"],"prefix":"10.1128","volume":"4","author":[{"given":"Nittaya","family":"Khakhum","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, 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