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The development of a safe, effective ZIKV vaccine is critical for several reasons: (i) the difficulty in diagnosing an active infection due to common nonspecific symptoms, (ii) the lack of a specific antiviral therapy, and (iii) the potentially devastating pathological effects of<jats:italic>in utero<\/jats:italic>infection. Moreover, a vaccine with an excellent safety profile, such as a nonreplicating, noninfectious vaccine, would be ideal for high-risk people (e.g., pregnant women, immunocompromised patients, and elderly individuals). This report describes the development of a recombinant subunit protein vaccine candidate derived from stably transformed insect cells expressing the ZIKV envelope protein<jats:italic>in vitro<\/jats:italic>, the primary antigen to which effective virus-neutralizing antibodies are engendered by immunized animals for several other flaviviruses; the vaccine candidate elicits effective virus-neutralizing antibodies against ZIKV and provides protection against ZIKV infection in mice.<\/jats:p>","DOI":"10.1128\/msphere.00576-17","type":"journal-article","created":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T10:39:07Z","timestamp":1515407947000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Recombinant Zika Virus Subunits Are Immunogenic and Efficacious in Mice"],"prefix":"10.1128","volume":"3","author":[{"given":"Albert","family":"To","sequence":"first","affiliation":[{"name":"Department of Tropical Medicine, Medical Microbiology, and Pharmacology, John A. 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