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Prevention of viral entry into susceptible cells is an attractive alternative strategy. Here we report that heparan sulfate-binding peptides effectively inhibit entry into fibroblasts of\n            <jats:italic>in vitro-<\/jats:italic>\n            derived CMVs and partially inhibit\n            <jats:italic>in vivo-<\/jats:italic>\n            derived CMVs. This includes the inhibition of urine-derived HCMV (uCMV), which is highly resistant to antibody neutralization. While these antiviral peptides are highly effective at inhibiting cell-free virus, they do not inhibit MCMV cell-to-cell spread. This underscores the need to understand the mechanism of cell-to-cell spread and differences between\n            <jats:italic>in vivo<\/jats:italic>\n            -derived versus\n            <jats:italic>in vitro-<\/jats:italic>\n            derived CMV entry to effectively prevent CMV\u2019s spread.\n          <\/jats:p>","DOI":"10.1128\/msphere.00586-18","type":"journal-article","created":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T11:30:10Z","timestamp":1549971010000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Anticytomegalovirus Peptides Point to New Insights for CMV Entry Mechanisms and the Limitations of\n            <i>In Vitro<\/i>\n            Screenings"],"prefix":"10.1128","volume":"4","author":[{"given":"Joseph W.","family":"Jackson","sequence":"first","affiliation":[{"name":"Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor J.","family":"Hancock","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pranay","family":"Dogra","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, USA"},{"name":"Columbia Center for Translational Immunology, Columbia University, New York City, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravi","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5363-167X","authenticated-orcid":false,"given":"Ravit","family":"Arav-Boger","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela D.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Tennessee Medical Center, Knoxville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen J.","family":"Kennel","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Tennessee Medical Center, Knoxville, Tennessee, USA"},{"name":"Department of Radiology, The University of Tennessee Medical Center, Knoxville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan S.","family":"Wall","sequence":"additional","affiliation":[{"name":"Department of Medicine, The University of Tennessee Medical Center, Knoxville, Tennessee, USA"},{"name":"Department of Radiology, The University of Tennessee Medical Center, Knoxville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim E.","family":"Sparer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Human herpesviruses: biology, therapy, and immunoprophylaxis","author":"Britt W","year":"2007","unstructured":"Britt W. 2007. Virus entry into host, establishment of infection, spread in host, mechanisms of tissue damage. In Arvin A, Campadelli-Fiume G, Mocarski E, Moore PS, Roizman B, Whitley R, Yamanishi K (ed), Human herpesviruses: biology, therapy, and immunoprophylaxis. Cambridge University Press, Cambridge, United Kingdom."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu346"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81891-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/508173"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00062-12"},{"key":"e_1_3_2_7_2","first-page":"36","article-title":"Congenital cytomegalovirus infection","volume":"1","author":"Demmler GJ","year":"1994","unstructured":"Demmler GJ. 1994. Congenital cytomegalovirus infection. 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