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In each case, the development of disease and the degree of tissue damage are mediated by toxins released from the bacteria during infection. Consequently, novel therapies aimed at clearing bacterial toxins are greatly needed. One promising new treatment is the utilization of monoclonal antibodies delivered as an immunotherapeutic for toxin neutralization. However, current methods of antibody development are laborious and costly. Here, we report a method to enrich and increase the detection of highly desirable antigen-specific memory B cells from individuals previously exposed to GAS using a cost-effective and less-time-intensive strategy. We envision that this method will be incorporated into many applications supporting the development of immunotherapeutics.<\/jats:p>","DOI":"10.1128\/msphere.00598-19","type":"journal-article","created":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T14:13:51Z","timestamp":1572963231000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Enrichment of Antigen-Specific Class-Switched B Cells from Individuals Naturally Immunized by Infection with Group A Streptococcus"],"prefix":"10.1128","volume":"4","author":[{"given":"Cheri L.","family":"Lamb","sequence":"first","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"},{"name":"Idaho Veterans Research and Education Foundation, Boise, Idaho, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Price","sequence":"additional","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"},{"name":"Idaho Veterans Research and Education Foundation, Boise, Idaho, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P.","family":"Field","sequence":"additional","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"},{"name":"Idaho Veterans Research and Education Foundation, Boise, Idaho, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Dayton","sequence":"additional","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"},{"name":"Idaho Veterans Research and Education Foundation, Boise, Idaho, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric R.","family":"McIndoo","sequence":"additional","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva J.","family":"Katahira","sequence":"additional","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis L.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"},{"name":"Idaho Veterans Research and Education Foundation, Boise, Idaho, USA"},{"name":"University of Washington School of Medicine, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Hobdey","sequence":"additional","affiliation":[{"name":"Infectious Diseases Section, Veteran Affairs Medical Center, Boise, Idaho, USA"},{"name":"Idaho Veterans Research and Education Foundation, Boise, Idaho, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd984"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2747"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2016.1269580"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2015.1125583"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4161\/19420862.2015.988944"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00101-13"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-341917"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2017.05.010"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5284.94"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.102"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.2337\/db13-1798"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.3791\/55382"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091014-104233"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2033520100"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/432729"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000266"},{"key":"e_1_3_2_18_2","volume-title":"Acute reversible cardiomyopathy in patients with streptococcal toxic shock syndrome","author":"Stevens DL","year":"2008","unstructured":"Stevens DL, Shelly M, Stiller R, Villasenor-Sierra SD, Bryant AE. 2008. 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