{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:43:31Z","timestamp":1753883011308,"version":"3.41.2"},"reference-count":5,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T00:00:00Z","timestamp":1567555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,10,30]],"date-time":"2019-10-30T00:00:00Z","timestamp":1572393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["208780\/Z\/17\/Z"],"award-info":[{"award-number":["208780\/Z\/17\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2019,10,30]]},"abstract":"<jats:p>\n            Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan\n            <jats:named-content content-type=\"genus-species\">Trypanosoma brucei<\/jats:named-content>\n            . In this\n            <jats:italic>mSphere of Influence<\/jats:italic>\n            article, he reflects on how the paper \u201cComprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)\u201d by Queiroz et al. (Nat Biotechnol 37:169\u2013178, 2019,\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1038\/s41587-018-0001-2\">https:\/\/doi.org\/10.1038\/s41587-018-0001-2<\/jats:ext-link>\n            ) influenced his research by providing a tool to capture RNA-protein complexes on a global scale using acid guanidinium thiocyanate-phenol-chloroform (AGPC), an old method hitherto applied for RNA, DNA, or protein purification.\n          <\/jats:p>","DOI":"10.1128\/msphere.00600-19","type":"journal-article","created":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T12:42:06Z","timestamp":1567514526000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions"],"prefix":"10.1128","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3742-6502","authenticated-orcid":false,"given":"Calvin","family":"Tiengwe","sequence":"first","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-018-0001-2"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1980.tb07207.x"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.11.004"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08942-3"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20188689"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00600-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00600-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T04:26:13Z","timestamp":1646713573000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00600-19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,30]]},"references-count":5,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,10,30]]}},"alternative-id":["10.1128\/mSphere.00600-19"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00600-19","relation":{},"ISSN":["2379-5042"],"issn-type":[{"type":"electronic","value":"2379-5042"}],"subject":[],"published":{"date-parts":[[2019,10,30]]},"assertion":[{"value":"2019-09-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00600-19"}}