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The latter is usually fatal if not treated. Persistence of<jats:named-content content-type=\"genus-species\">L. major<\/jats:named-content>in the mammalian host depends upon maintaining gene-regulatory programs to support essential parasite metabolic functions. These include expression and assembly of mitochondrial<jats:italic>L. major<\/jats:italic>cytochrome<jats:italic>c<\/jats:italic>oxidase (LmCOX) subunits, important for<jats:italic>Leishmania<\/jats:italic>ATP production. Significantly, under mammalian conditions, WT levels of LmCOX subunits require threshold levels of the<jats:italic>Leishmania<\/jats:italic>ribosome-associated scaffold protein, LACK. Unexpectedly, we find that although disruption of LACK\u2019s putative ribosome-binding motif does not grossly perturb ribosome association or global protein synthesis, it nonetheless impairs COX subunit expression, mitochondrial function, and virulence. Our data indicate that the quality of LACK\u2019s interaction with<jats:italic>Leishmania<\/jats:italic>ribosomes is critical for LmCOX subunit expression and parasite mitochondrial function in the mammalian host. Collectively, these findings validate LACK\u2019s ribosomal interactions as a potential therapeutic target.<\/jats:p>","DOI":"10.1128\/msphere.00644-18","type":"journal-article","created":{"date-parts":[[2019,3,5]],"date-time":"2019-03-05T11:21:34Z","timestamp":1551784894000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Disruption of the Putative Ribosome-Binding Motif of a Scaffold Protein Impairs Cytochrome<i>c<\/i>Oxidase Subunit Expression in<i>Leishmania major<\/i>"],"prefix":"10.1128","volume":"4","author":[{"given":"Daviel","family":"Cardenas","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charity","family":"Sylvester","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology and Tulane Cancer Center, Tulane University School of Medicine, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine S.","family":"Nation","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Tulane University, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Pizarro","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, Tulane University, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology and Tulane Cancer Center, Tulane University School of Medicine, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessie","family":"Guidry","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J.","family":"Wojcik","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben L.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9254com"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004300100075"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.07.007"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-157529"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031162"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12924"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020710"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01718-08"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb822"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-05-0388"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4130376"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58488"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13223"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2014.08.015"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.11154"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"5504","DOI":"10.4049\/jimmunol.151.10.5504","article-title":"Trypanosoma cruzi acidic ribosomal P protein gene family. 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