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Despite this commonality, our understanding of the role of most glycosylation systems in bacterial physiology and pathogenesis is incomplete. In this work, we investigated the effect of the disruption of\n            <jats:italic>O<\/jats:italic>\n            -linked glycosylation in the opportunistic pathogen\n            <jats:named-content content-type=\"genus-species\">Burkholderia cenocepacia<\/jats:named-content>\n            using a combination of proteomic, molecular, and phenotypic assays. We find that in contrast to recent findings on the\n            <jats:italic>N<\/jats:italic>\n            -linked glycosylation systems of\n            <jats:named-content content-type=\"genus-species\">Campylobacter jejuni<\/jats:named-content>\n            ,\n            <jats:italic>O<\/jats:italic>\n            -linked glycosylation does not appear to play a role in proteome stabilization of most glycoproteins. Our results reveal that loss of glycosylation in\n            <jats:named-content content-type=\"genus-species\">B. cenocepacia<\/jats:named-content>\n            strains leads to global proteome and transcriptional changes, including the repression of the quorum-sensing regulator\n            <jats:italic>cepR<\/jats:italic>\n            (\n            <jats:italic>BCAM1868<\/jats:italic>\n            ) gene. These alterations lead to dramatic phenotypic changes in glycosylation-null strains, which are paralleled by both global proteomic and transcriptional alterations, which do not appear to directly result from the loss of glycosylation per se. This research unravels the pleiotropic effects of\n            <jats:italic>O<\/jats:italic>\n            -linked glycosylation in\n            <jats:named-content content-type=\"genus-species\">B. cenocepacia<\/jats:named-content>\n            , demonstrating that its loss does not simply affect the stability of the glycoproteome, but also interferes with transcription and the broader proteome.\n          <\/jats:p>","DOI":"10.1128\/msphere.00660-19","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T11:05:58Z","timestamp":1573556758000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Loss of\n            <i>O<\/i>\n            -Linked Protein Glycosylation in Burkholderia cenocepacia Impairs Biofilm Formation and Siderophore Activity and Alters Transcriptional Regulators"],"prefix":"10.1128","volume":"4","author":[{"given":"Cameron 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