{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T06:48:57Z","timestamp":1786171737074,"version":"build-2736575974"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["179310, 207917"],"award-info":[{"award-number":["179310, 207917"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2024,7,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Botrytis cinerea<\/jats:italic>\n              is a necrotrophic phytopathogen able to attack more than 200 different plant species causing strong yield losses worldwide. Many synthetic fungicides have been developed to control this disease, resulting in the rise of fungicide-resistance\n              <jats:italic>B. cinerea<\/jats:italic>\n              strains. The aim of this study was to identify\n              <jats:italic>Streptomyces<\/jats:italic>\n              strains showing antagonistic activity against\n              <jats:italic>B. cinerea<\/jats:italic>\n              to contribute to plant protection in an environmentally friendly way. We isolated 15 Actinomycete strains from 9 different Swiss soils. The culture filtrates of three isolates showing antifungal activity inhibited spore germination and delayed mycelial growth of\n              <jats:italic>B. cinerea<\/jats:italic>\n              . Infection experiments showed that\n              <jats:italic>Arabidopsis thaliana<\/jats:italic>\n              plants were more resistant to this pathogen after leaf treatment with the\n              <jats:italic>Streptomyces<\/jats:italic>\n              filtrates. Bioassay-guided isolation of the active compounds revealed the presence of germicidins A and B as well as of oligomycins A, B, and E. While germicidins were mostly inactive, oligomycin B reduced the mycelial growth of\n              <jats:italic>B. cinerea<\/jats:italic>\n              significantly. Moreover, all three oligomycins inhibited this fungus\u2019 spore germination, suggesting that these molecules might contribute to the\n              <jats:italic>Streptomyces<\/jats:italic>\n              \u2019s ability to protect plants against infection by the broad host-pathogen\n              <jats:italic>Botrytis cinerea<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              This study reports the isolation of new\n              <jats:italic>Streptomyces<\/jats:italic>\n              strains with strong plant-protective potential mediated by their production of specialized metabolites. Using the broad host range pathogenic fungus\n              <jats:italic>Botrytis cinerea<\/jats:italic>\n              , we demonstrate that the cell-free filtrate of selected\n              <jats:italic>Streptomyces<\/jats:italic>\n              isolates efficiently inhibits different developmental stages of the fungus, including mycelial growth and the epidemiologically relevant spore germination. Beyond\n              <jats:italic>in vitro<\/jats:italic>\n              experiments, the strains and their metabolites also efficiently protected plants against the disease caused by this pathogen. This work further identifies oligomycins as active compounds involved in the observed antifungal activity of the strains. This work shows that we can harness the natural ability of soil-borne microbes and of their metabolites to efficiently fight other microbes responsible for significant crop losses. This opens the way to the development of environmentally friendly health protection measures for crops of agronomical relevance, based on these newly isolated strains or their metabolic extracts containing oligomycins.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00667-23","type":"journal-article","created":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T13:02:31Z","timestamp":1718197351000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Oligomycin-producing\n            <i>Streptomyces<\/i>\n            sp. newly isolated from Swiss soils efficiently protect\n            <i>Arabidopsis thaliana<\/i>\n            against\n            <i>Botrytis cinerea<\/i>"],"prefix":"10.1128","volume":"9","author":[{"given":"Fanny","family":"Louviot","sequence":"first","affiliation":[{"name":"Department of Biology, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1886-6366","authenticated-orcid":true,"given":"Ola","family":"Abdelrahman","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliane","family":"Abou-Mansour","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Floriane","family":"L'Haridon","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Marie","family":"Allard","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Falquet","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Fribourg, Fribourg, Switzerland"},{"name":"Swiss Institute of Bioinformatics, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8789-3830","authenticated-orcid":true,"given":"Laure","family":"Weisskopf","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Fribourg, Fribourg, Switzerland"},{"name":"Food Research and Innovation Centre, University of Fribourg, Fribourg, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2007.00417.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2011.00783.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2011.06.013"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy10111822"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1094\/PD-71-0799"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1049-9644(02)00051-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028x-63.3.386"},{"key":"e_1_3_3_9_2","first-page":"21","article-title":"Biological control of grey mould of stored table grapes by pre-harvest applications of Aureobasidium pullulans and Candida oleophila","volume":"20","author":"Lima G","year":"1997","unstructured":"Lima G, Ippolito A, Nigro F, Salerno M. 1997. 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