{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T13:26:07Z","timestamp":1783776367226,"version":"3.55.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T00:00:00Z","timestamp":1597190400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000995","name":"Australian National University","doi-asserted-by":"publisher","award":["PhD Scholarship"],"award-info":[{"award-number":["PhD Scholarship"]}],"id":[{"id":"10.13039\/501100000995","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Department of Education and Training | Australian Research Council","doi-asserted-by":"publisher","award":["DP180103212"],"award-info":[{"award-number":["DP180103212"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007727","name":"Department of Industry, Innovation and Science, Australian Government","doi-asserted-by":"publisher","award":["Research Training Program Scholarship"],"award-info":[{"award-number":["Research Training Program Scholarship"]}],"id":[{"id":"10.13039\/501100007727","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,8,26]]},"abstract":"<jats:p>\n            The protozoan\n            <jats:named-content content-type=\"genus-species\">Plasmodium falciparum<\/jats:named-content>\n            causes the most severe form of human malaria. The development of sexual forms (so-called gametocytes) is crucial for disease transmission. However, knowledge of these forms is severely hampered by the paucity of sex-specific markers and the inability to extract single sex gametocytes in high purity. Moreover, the identification of compounds that specifically affect one sex is difficult due to the female bias of the gametocytes. We have developed a system that allows for the separation of male and female gametocytes from the same population. Applying our system, we show that male and female parasites mature at different rates, which might have implications for transmission. We also identified new sex-specific genes that can be used as sex markers or to unravel sex-specific functions. Our system will not only aid in the discovery of much needed gametocidal compounds, but it also represents a valuable tool for exploring malaria transmission biology.\n          <\/jats:p>","DOI":"10.1128\/msphere.00671-20","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T13:45:20Z","timestamp":1597758320000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Novel Method for the Separation of Male and Female Gametocytes of the Malaria Parasite\n            <i>Plasmodium falciparum<\/i>\n            That Enables Biological and Drug Discovery"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6763-2366","authenticated-orcid":false,"given":"Melanie C.","family":"Ridgway","sequence":"first","affiliation":[{"name":"Research School of Biology, The Australian National University, Canberra, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwong Sum","family":"Shea","sequence":"additional","affiliation":[{"name":"Research School of Biology, The Australian National University, Canberra, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Cihalova","sequence":"additional","affiliation":[{"name":"Research School of Biology, The Australian National University, Canberra, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7369-1058","authenticated-orcid":false,"given":"Alexander G.","family":"Maier","sequence":"additional","affiliation":[{"name":"Research School of Biology, The Australian National University, Canberra, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182000047478"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-1986-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000406"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(13)70002-0"},{"key":"e_1_3_2_6_2","volume-title":"World malaria report\u20142018","author":"World Health Organization","year":"2018","unstructured":"World Health Organization. 2018. 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