{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T17:19:10Z","timestamp":1793035150967,"version":"4.5.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["U19AI116482-01"],"award-info":[{"award-number":["U19AI116482-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI13540"],"award-info":[{"award-number":["R21AI13540"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["T32AI007528"],"award-info":[{"award-number":["T32AI007528"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2024,12,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Nontyphoidal strains of\n              <jats:italic>Salmonella enterica<\/jats:italic>\n              are a major cause of foodborne illnesses, and infection with these bacteria results in inflammatory gastroenteritis. Polymorphonuclear leukocytes (PMNs), also known as neutrophils, are a dominant immune cell type found at the site of infection in\n              <jats:italic>Salmonella-<\/jats:italic>\n              infected individuals, but how they regulate infection outcome is not well understood. Here, we used a co-culture model of primary human PMNs and human intestinal organoids to probe the role of PMNs during infection with two of the most prevalent\n              <jats:italic>Salmonella<\/jats:italic>\n              serovars:\n              <jats:italic>Salmonella enterica<\/jats:italic>\n              serovar Enteritidis and Typhimurium. Using a transcriptomics approach, we identified a dominant role for PMNs in mounting differential immune responses including production of pro-inflammatory cytokines, chemokines, and antimicrobial peptides. We also identified specific gene sets that were induced by PMNs in response to Enteritidis or Typhimurium infection. By comparing host responses to these serovars, we uncovered differential regulation of host metabolic pathways particularly induction of cholesterol biosynthetic pathways during Typhimurium infection and suppression of RNA metabolism during Enteritidis infection. Together, these findings provide insight into the role of human PMNs in modulating different host responses to pathogens that cause similar disease in humans.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Nontyphoidal serovars of\n                <jats:italic>Salmonella enterica<\/jats:italic>\n                are known to induce robust recruitment of polymorphonuclear leukocytes (PMNs) in the gut during early stages of infection, but the specific role of PMNs in regulating infection outcome of different serovars is poorly understood. Due to differences in human infection progression compared to small animal models, characterizing the role of PMNs during infection has been challenging. Here, we used a co-culture model of human intestinal organoids with human primary PMNs to study the role of PMNs during infection of human intestinal epithelium. Using a transcriptomics approach, we define PMN-dependent reprogramming of the host response to\n                <jats:italic>Salmonella<\/jats:italic>\n                , establishing a clear role in amplifying pro-inflammatory gene expression. Additionally, the host response driven by PMNs differed between two similar nontyphoidal\n                <jats:italic>Salmonella<\/jats:italic>\n                serovars. These findings highlight the importance of building more physiological infection models to replicate human infection conditions to study host responses specific to individual pathogens.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00693-24","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T14:00:37Z","timestamp":1732111237000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Neutrophil prime unique transcriptional responses in intestinal organoids during infection with nontyphoidal\n            <i>Salmonella enterica<\/i>\n            serovars"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2865-0238","authenticated-orcid":false,"given":"Anna-Lisa E.","family":"Lawrence","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan P.","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Hill","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious Diseases Division, University of Michigan Medical School","place":["Ann Arbor, USA"]},{"name":"Department of Cell and Developmental Biology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veda K.","family":"Yadagiri","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious Diseases Division, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brooke","family":"Bons","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious Diseases Division, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney","family":"Fields","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious Diseases Division, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason S.","family":"Knight","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine\/Infectious Diseases Division, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5286-0924","authenticated-orcid":true,"given":"Christiane E.","family":"Wobus","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason R.","family":"Spence","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3687-2364","authenticated-orcid":true,"given":"Vincent B.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School","place":["Ann Arbor, USA"]},{"name":"Department of Internal Medicine\/Infectious Diseases Division, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6492-2707","authenticated-orcid":true,"given":"Basel H.","family":"Abuaita","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6014-4637","authenticated-orcid":true,"given":"Mary X.","family":"O'Riordan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School","place":["Ann Arbor, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","unstructured":"CDC. 2020. Foodborne germs and illnesses. Available from: https:\/\/www.cdc.gov\/foodsafety\/foodborne-germs.html"},{"key":"e_1_3_5_3_2","unstructured":"Salmonella homepage. 2021. Available from: https:\/\/www.cdc.gov\/salmonella"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92844-8"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP-0007-2018"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2005.08.008"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00399-21"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00161-15"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009987"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09691"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.29132"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178966"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01470-20"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03348-19"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006532"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003668"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.9.5789"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00051.x"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-020711-074942"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.11.020"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.24"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","unstructured":"Lawrence A-L Berger RP Hill DR Huang S Yadagiri VK Bons B Fields C Sule GJ Knight JS Wobus CE Spence JR Young VB O\u2019Riordan MX Abuaita BH. 2022. Human neutrophils direct epithelial cell extrusion to enhance intestinal epithelial host defense during Salmonella infection. Microbiology. doi:10.1101\/2022.03.25.485742","DOI":"10.1101\/2022.03.25.485742"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baw100"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.09.001"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.6.1197"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115800"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116462"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115156"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.4161\/21688362.2014.969100"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01270"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2009.08.003"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-018-0257-6"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05497-11"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148706"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.284"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5540-2"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2021-324855"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(99)01004-2"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115253"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.7"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06142.x"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13329"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.1036-1042.2004"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003107"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-11-152"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms150712558"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1706070114"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2001321"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","unstructured":"Abuaita BH Lawrence A-L Berger RP Hill DR Huang S Yadagiri VK Bons B Fields C Wobus CE Spence JR Young VB O\u2019Riordan MX. 2020. Comparative transcriptional profiling of the early host response to infection by typhoidal and non-typhoidal Salmonella serovars in human intestinal organoids. Microbiology. doi:10.1101\/2020.11.25.397620","DOI":"10.1101\/2020.11.25.397620"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.3791\/56960"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3519"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7563.2"},{"key":"e_1_3_5_53_2","unstructured":"Rainer J. 2017. EnsDb.Hsapiens.v75: Ensembl based annotation package"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_3_5_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1039\/c5mb00663e"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_3_5_58_2","volume-title":"R package version 0 4 3","author":"Wickham H","year":"2015","unstructured":"WickhamH, Francois R, HenryL, M\u00fcllerK. 2015. Dplyr: a grammar of data manipulation. In R package version 0 4 3"},{"key":"e_1_3_5_59_2","unstructured":"Larsson J. 2019. Eulerr: area-proportional euler and venn diagrams with ellipses"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00693-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00693-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T14:02:35Z","timestamp":1734616955000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00693-24"}},"subtitle":[],"editor":[{"given":"Sarah E. F.","family":"D'Orazio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,19]]},"references-count":58,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12,19]]}},"alternative-id":["10.1128\/msphere.00693-24"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00693-24","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,19]]},"assertion":[{"value":"2024-08-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00693-24"}}