{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:47:34Z","timestamp":1790858854472,"version":"4.1.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T00:00:00Z","timestamp":1596585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI119558"],"award-info":[{"award-number":["AI119558"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI26667"],"award-info":[{"award-number":["AI26667"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,8,26]]},"abstract":"<jats:p>\n            Colonization of the lung by\n            <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n            is a critical first step in establishing infection during primary pneumonic plague, a disease characterized by high lethality. However, the mechanisms by which\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            adheres in the lung after inhalation remain elusive. Here, we used Tn-seq to identify\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            genes important for adherence early during primary pneumonic plague. Our mutant enrichment strategy resulted in the identification of genes important for regulation and assembly of genes and proteins rather than adhesin genes themselves. These results reveal that there may be multiple\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            adhesins or redundancy among adhesins. Identifying the adhesins regulated by the genes identified in our enrichment screen may reveal novel therapeutic targets for preventing\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            adherence and the subsequent development of pneumonic plague.\n          <\/jats:p>","DOI":"10.1128\/msphere.00715-20","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T13:40:42Z","timestamp":1596548442000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Tn-Seq Analysis Identifies Genes Important for Yersinia pestis Adherence during Primary Pneumonic Plague"],"prefix":"10.1128","volume":"5","author":[{"given":"Kara R.","family":"Eichelberger","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2085-9667","authenticated-orcid":false,"given":"Victoria E.","family":"Sep\u00falveda","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Ford","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA"},{"name":"Department of Genetics, University of North Carolina, Chapel Hill, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara R.","family":"Selitsky","sequence":"additional","affiliation":[{"name":"Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, USA"},{"name":"Department of Genetics, University of North 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