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Despite this, little is known about how this fungus causes disease. Here, we applied RNA sequencing and an\n              <jats:italic>in vivo<\/jats:italic>\n              invasive infection model to identify the attributes that allow this organism to infect hosts. Fungal transcriptomes show a dramatic increase in the expression of Fus3 and Kss1, two mitogen-activated protein kinases (MAPKs), during invasive infection. We further demonstrate that they are both highly induced under a combination of serum and high CO\n              <jats:sub>2<\/jats:sub>\n              conditions. Deletion of both\n              <jats:italic>FUS3<\/jats:italic>\n              and\n              <jats:italic>KSS1<\/jats:italic>\n              , but neither gene alone, results in a reduced fungal burden in organs, as well as in the gastrointestinal tract in the DSS (Dextran Sulfate Sodium)-induced colitis model. Similarly, the defect in persistence in macrophages and attenuated adhesion to epithelial cells are observed when\n              <jats:italic>FUS3<\/jats:italic>\n              and\n              <jats:italic>KSS1<\/jats:italic>\n              are both disrupted. The\n              <jats:italic>fus3 kss1<\/jats:italic>\n              double mutant also displays defects in the induction of virulence attributes such as genes required for iron acquisition and adhesion and in the anti-fungal drug tolerance. The putative downstream transcription factors Ste12 (1), Ste12 (2), Tec1, and Tec2 are found to be involved in the regulation of these virulence attributes. Collectively, our study indicates that an evolutionary conserved MAPK pathway, which regulates mating and filamentous growth in\n              <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n              , is critical for\n              <jats:italic>C. glabrata<\/jats:italic>\n              pathogenicity.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The MAPK signaling pathway, mediated by closely related kinases Fus3 and Kss1, is crucial for controlling mating and filamentous growth in\n              <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n              , but this pathway does not significantly impact hyphal development and pathogenicity in\n              <jats:italic>Candida albicans<\/jats:italic>\n              , a commensal-pathogenic fungus of humans. Furthermore, deletion of Cpk1, the ortholog of Fus3 in pathogenic fungus\n              <jats:italic>Cryptococcus neoformans<\/jats:italic>\n              , has no effect on virulence. Here, we demonstrate that the MAPK pathway is crucial for the pathogenicity of\n              <jats:italic>Candida glabrata<\/jats:italic>\n              , a fungus that causes approximately one-third of cases of hematogenously disseminated candidiasis in the United States. This pathway regulates multiple virulence attributes including the induction of iron acquisition genes and adhesins, as well as persistence in macrophages and organs. Our work provides insights into\n              <jats:italic>C. glabrata<\/jats:italic>\n              pathogenesis and highlights an example in which regulatory rewiring of a conserved pathway confers a virulent phenotype in a pathogen.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00715-24","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T15:21:28Z","timestamp":1730301688000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["<i>In vivo<\/i>\n            RNA sequencing reveals a crucial role of Fus3-Kss1 MAPK pathway in\n            <i>Candida glabrata<\/i>\n            pathogenicity"],"prefix":"10.1128","volume":"9","author":[{"given":"Xinreng","family":"Mo","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangtai","family":"Yu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8774-5325","authenticated-orcid":false,"given":"Hao","family":"Cui","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Yang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4937-3249","authenticated-orcid":false,"given":"Chang","family":"Su","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3784-7577","authenticated-orcid":true,"given":"Yang","family":"Lu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, 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