{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:32:40Z","timestamp":1790793160260,"version":"4.1.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,1,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n              (\n              <jats:italic>P. gingivalis<\/jats:italic>\n              ), a gram-negative, black-pigmented anaerobe, is a major etiological agent and a leading cause of periodontitis. Fimbriae protein Mfa1 is a key virulence factor of\n              <jats:italic>P. gingivalis<\/jats:italic>\n              and plays a crucial role in bacterial adhesion, colonization, biofilm formation, and persistent inflammation, making it a promising therapeutic target. However, the role of anti-Mfa1 antibodies and the underlying protective mechanisms remain largely unexplored. Here, we developed and characterized the monoclonal antibodies (mAbs) targeting the Mfa1 protein of\n              <jats:italic>P. gingivalis<\/jats:italic>\n              . Function analysis showed that anti-Mfa1 mAbs mediated bacterial agglutination and inhibited\n              <jats:italic>P. gingivalis<\/jats:italic>\n              adhesion to saliva-coated hydroxyapatite and host cells. Notably, anti-Mfa1 mAbs significantly reduced bacterial burden and alveolar bone loss in a\n              <jats:italic>P. gingivalis<\/jats:italic>\n              -induced experimental periodontitis model. These results show that anti-Mfa1 mAbs can be beneficial in alleviating\n              <jats:italic>P. gingivalis<\/jats:italic>\n              infections, and provide important insights for the development of adequate adjuvant treatment regimens for Mfa1-targeted therapeutics.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Fimbriae (pili) play an important role in bacterial adhesion, invasion of host cells and tissues, and formation of biofilms. Studies have shown that two types of fimbriae of\n              <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n              , FimA and Mfa1, are important for colonization and infection through their binding to host tissues and other bacteria. While anti-FimA antibodies have been shown to improve periodontitis, the effect of anti-Mfa1 antibodies on\n              <jats:italic>P. gingivalis<\/jats:italic>\n              infection and periodontitis was previously unknown. In this study, we report for the first time that anti-Mfa1 monoclonal antibodies can reduce\n              <jats:italic>P. gingivalis<\/jats:italic>\n              infection and improve periodontitis. These findings suggest that Mfa1 represents a promising therapeutic target, and the development of anti-Mfa1 mAbs holds a potential as essential diagnostic and adjunctive therapeutic tools for managing\n              <jats:italic>P. gingivalis<\/jats:italic>\n              -related diseases.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00721-24","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T14:04:40Z","timestamp":1734617080000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Development of monoclonal antibodies against\n            <i>P. gingivalis<\/i>\n            Mfa1 and their protective capacity in an experimental periodontitis model"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9911-3991","authenticated-orcid":false,"given":"Mingya","family":"Cao","sequence":"first","affiliation":[{"name":"Joint National Laboratory for Antibody Drug Engineering, School of Medicine, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Joint National Laboratory for Antibody Drug Engineering, School of Medicine, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengke","family":"Zhou","sequence":"additional","affiliation":[{"name":"Joint National Laboratory for Antibody Drug Engineering, School of Medicine, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yan","sequence":"additional","affiliation":[{"name":"Joint National Laboratory for Antibody Drug Engineering, School of Medicine, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zou","sequence":"additional","affiliation":[{"name":"Joint National Laboratory for Antibody Drug Engineering, School of Medicine, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Cui","sequence":"additional","affiliation":[{"name":"Joint National Laboratory for Antibody Drug Engineering, School of Medicine, Henan University","place":["Kaifeng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Lou","sequence":"additional","affiliation":[{"name":"Joint National Laboratory for Antibody Drug Engineering, School of Medicine, Henan University","place":["Kaifeng, 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