{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:54:07Z","timestamp":1791500047999,"version":"4.3.4"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["AI150797"],"award-info":[{"award-number":["AI150797"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2024,3,26]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Brucellosis, caused by the bacterium\n              <jats:italic>Brucella<\/jats:italic>\n              , poses a significant global threat to both animal and human health. Although commercial live\n              <jats:italic>Brucella<\/jats:italic>\n              vaccines including S19, RB51, and Rev1 are available for animals, their unsuitability for human use and incomplete efficacy in animals necessitate the further study of vaccine-mediated immunity to\n              <jats:italic>Brucella<\/jats:italic>\n              . In this study, we employed\n              <jats:italic>in vivo<\/jats:italic>\n              B-cell depletion, as well as immunodeficient and transgenic mouse models, to comprehensively investigate the roles of B cells, antigen uptake and presentation, antibody production, and class switching in the context of S19-mediated immunity against brucellosis. We found that antibody production, and in particular secretory IgM plays a protective role in S19-mediated immunity against virulent\n              <jats:italic>Brucella melitensis<\/jats:italic>\n              early after the challenge in a manner associated with complement activation. While T follicular helper cell deficiency dampened IgG production and vaccine efficacy at later stages of the challenge, this effect appeared to be independent of antibody production and rather was associated with altered T-cell function. By contrast, B-cell MHCII expression negatively impacted vaccine efficacy at later timepoints after the challenge. In addition, B-cell depletion after vaccination, but before the challenge, enhanced S19-mediated protection against brucellosis, suggesting a deleterious role of B cells during the challenge phase. Collectively, our findings indicate antibody production is protective, while B-cell MHCII expression is deleterious, to live vaccine-mediated immunity against brucellosis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Brucella<\/jats:italic>\n              is a neglected zoonotic pathogen with a worldwide distribution. Our study delves into B-cell effector functions in live vaccine-mediated immunity against brucellosis. Notably, we found antibody production, particularly secretory IgM, confers protection against virulent\n              <jats:italic>Brucella melitensis<\/jats:italic>\n              in vaccinated mice, which was associated with complement activation. By contrast, B-cell MHCII expression negatively impacted vaccine efficacy. In addition, B-cell depletion after vaccination, but before the\n              <jats:italic>B. melitensis<\/jats:italic>\n              challenge, enhanced protection against infection, suggesting a detrimental B-cell role during the challenge phase. Interestingly, deficiency of T follicular helper cells, which are crucial for aiding germinal center B cells, dampened vaccine efficacy at later stages of challenge independent of antibody production. This study underscores contrasting and phase-dependent roles of B-cell effector functions in vaccine-mediated immunity against\n              <jats:italic>Brucella<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00750-23","type":"journal-article","created":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T14:01:15Z","timestamp":1707832875000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Contrasting roles for IgM and B-cell MHCII expression in\n            <i>Brucella abortus<\/i>\n            S19 vaccine-mediated efficacy against\n            <i>B. melitensis<\/i>\n            infection"],"prefix":"10.1128","volume":"9","author":[{"given":"Mostafa F. N.","family":"Abushahba","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri, Columbia, Missouri, USA"},{"name":"Department of Zoonoses, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis S.","family":"Dadelahi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e1rbara","family":"Ponzilacqua-Silva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles R.","family":"Moley","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2337-908X","authenticated-orcid":true,"given":"Jerod A.","family":"Skyberg","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA"},{"name":"Laboratory for Infectious Disease Research, University of Missouri, Columbia, Missouri, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-017-5016-y"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020186"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.03.003"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2909.230052"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.23.01.0033"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13690-017-0207-7"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.radcr.2022.07.012"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2022.e12134"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-05002-2_15"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.07.007"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-003-1026-z"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed3020065"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2017.12.006"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.903890"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1018165"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiq171"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00761-12"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00075-20"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302561"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01589"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2016.10.006"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.11.3438-3444.1989"},{"key":"e_1_3_4_24_2","first-page":"651","article-title":"Antibody-mediated protection against Brucella abortus in BALB\/C mice at successive periods after infection: variation between virulent strain 2308 and attenuated vaccine strain 19","volume":"82","author":"Elzer PH","year":"1994","unstructured":"Elzer PH, Jacobson RH, Jones SM, Nielsen KH, Douglas JT, Winter AJ. 1994. 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