{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:39:44Z","timestamp":1790401184687,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,10,28]]},"abstract":"<jats:p>\n            In many parts of the world, carbapenem resistance is a serious public health concern. In Brazil, carbapenem resistance in\n            <jats:italic>Enterobacterales<\/jats:italic>\n            is mostly driven by the dissemination of KPC-2-producing\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            clones. Despite being endemic in this country, only a few reports providing both clinical and genomic data are available in Brazil, which limit the understanding of the real clinical impact caused by the dissemination of different clones carrying\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            in Brazilian hospitals. Although several of these KPC-2-producer\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            isolates belong to the clonal complex 258 and carry Tn\n            <jats:italic>4401<\/jats:italic>\n            transposons located on large plasmids, a concomitant emergence and silent dissemination of small high-copy-number\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC-2<\/jats:sub>\n            plasmids are of importance, as described in this study. Our data identify a small high-copy-number IncQ1 KPC plasmid, its clinical relevance, and the potential for conjugative transfer into several\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            isolates, belonging to different international lineages, such as ST258, ST101, and ST15.\n          <\/jats:p>","DOI":"10.1128\/msphere.00756-20","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T14:33:56Z","timestamp":1601994836000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Clinical and Molecular Description of a High-Copy IncQ1 KPC-2 Plasmid Harbored by the International ST15 Klebsiella pneumoniae Clone"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3001-3625","authenticated-orcid":false,"given":"Willames M. B. 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