{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T02:38:50Z","timestamp":1787625530105,"version":"build-2736575974"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,10,28]]},"abstract":"<jats:p>\n            The identification and characterization of regulators essential for virulence or development constitute one approach for antifungal drug development. In this study, we screened and functionally characterized\n            <jats:italic>Afmed15<\/jats:italic>\n            , a novel developmental regulator in\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            . We demonstrate that the precise transcriptional expression of\n            <jats:italic>Afmed15<\/jats:italic>\n            is crucial for fungal asexual development, virulence, and survival. Downregulating the expression of\n            <jats:italic>Afmed15<\/jats:italic>\n            abolished the conidiation and decreased the fungal virulence in an insect model. In contrast, the overexpression of\n            <jats:italic>Afmed15<\/jats:italic>\n            caused fungal death accompanied by intensive autophagy. Our study provides a foundation for further studies to identify compounds perturbing the expression of\n            <jats:italic>Afmed15<\/jats:italic>\n            that may be used for the prevention of invasive\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            infections.\n          <\/jats:p>","DOI":"10.1128\/msphere.00771-20","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T14:33:56Z","timestamp":1601994836000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Precise Expression of\n            <i>Afmed15<\/i>\n            Is Crucial for Asexual Development, Virulence, and Survival of Aspergillus fumigatus"],"prefix":"10.1128","volume":"5","author":[{"given":"Luyu","family":"Guan","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering 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