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Thus, they represent a significant cause of nosocomial morbidity and mortality. Yet how these organisms behave in the context of polymicrobial growth remains poorly understood. In this work, we set out to determine the mechanism by which activation of the staphylococcal\n            <jats:italic>agr<\/jats:italic>\n            quorum sensing system and production of its major virulence effector alpha-toxin is enhanced during coculture with\n            <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n            . Surprisingly, we likely ruled out that a secreted candidal factor drives this process. Instead, we demonstrated that alkalinization of the extracellular milieu by\n            <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n            and other\n            <jats:italic>Candida<\/jats:italic>\n            species correlated with elevated\n            <jats:italic>agr<\/jats:italic>\n            activity. Thus, we propose a mechanism where modulation of the extracellular pH by fungal opportunists can indirectly alter virulence of a bacterial pathogen. Uncovering molecular events that drive interkingdom pathogenicity mechanisms may enhance surveillance and treatment for devastating polymicrobial infections.\n          <\/jats:p>","DOI":"10.1128\/msphere.00780-19","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T11:05:58Z","timestamp":1573556758000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Candida albicans Impacts Staphylococcus aureus Alpha-Toxin Production via Extracellular Alkalinization"],"prefix":"10.1128","volume":"4","author":[{"given":"Olivia A.","family":"Todd","sequence":"first","affiliation":[{"name":"Integrated Program in Biomedical Sciences, Microbiology, Immunology, and Biochemistry track, University of Tennessee Health Science Center, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mairi C.","family":"Noverr","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Tulane University, New Orleans, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian M.","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacy and Translational Science, University of Tennessee Health Science Center, Memphis, Tennessee, USA"},{"name":"Department of Microbiology, Immunology, &amp; Biochemistry, University of Tennessee Health Science Center, Memphis, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2007.11.014"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.07.001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2007.06.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.081869"},{"key":"e_1_3_2_6_2","first-page":"119","article-title":"Methicillin-resistant Staphylococcus aureus and Candida albicans on denture surfaces","volume":"37","author":"Tawara Y","year":"1996","unstructured":"Tawara Y, Honma K, Naito Y. 1996. 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