{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:43:20Z","timestamp":1790639000219,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","award":["TI07.003\/80115MDMAW"],"award-info":[{"award-number":["TI07.003\/80115MDMAW"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,8,26]]},"abstract":"<jats:p>\n            Psittacosis, caused by avian\n            <jats:named-content content-type=\"genus-species\">C. psittaci<\/jats:named-content>\n            , has a major economic impact in the poultry industry worldwide and represents a significant risk for zoonotic transmission to humans. In the past decade, the tools of genetic manipulation have been improved for chlamydial molecular studies. While several genetic tools have been mainly developed in\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            , a stable gene-inducible shuttle vector system has not to date been available for\n            <jats:named-content content-type=\"genus-species\">C. psittaci<\/jats:named-content>\n            . In this study, we adapted a\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            plasmid shuttle vector system to\n            <jats:named-content content-type=\"genus-species\">C. psittaci<\/jats:named-content>\n            . We constructed a\n            <jats:named-content content-type=\"genus-species\">C. psittaci<\/jats:named-content>\n            plasmid backbone shuttle vector called pCps-Tet-mCherry. The construct expresses GFP in\n            <jats:named-content content-type=\"genus-species\">C. psittaci<\/jats:named-content>\n            . Importantly, exogeneous genes can be inserted at an MCS and are regulated by a tet promoter. The application of the pCps-Tet-mCherry shuttle vector system enables a promising new approach to investigate unknown gene functions of this pathogen.\n          <\/jats:p>","DOI":"10.1128\/msphere.00787-20","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T13:01:12Z","timestamp":1598360472000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5357-4230","authenticated-orcid":false,"given":"Kensuke","family":"Shima","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Microbiology, University of L\u00fcbeck, L\u00fcbeck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5126-2164","authenticated-orcid":false,"given":"Mary M.","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Schnee","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathogenesis, Friedrich-Loeffler-lnstitut (Federal Research Institute for Animal Health), Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1230-1786","authenticated-orcid":false,"given":"Konrad","family":"Sachse","sequence":"additional","affiliation":[{"name":"RNA Bioinformatics and High-Throughput Analysis, Faculty of Mathematics and Computer Science, Friedrich-Schiller-Universit\u00e4t Jena, Jena, 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