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Initially, the parasite grows rapidly, infecting and destroying cells of the host, but subsequently switches to a slow-growing form and establishes chronic infection. In both stages, the parasite lives within a membrane-bound vacuole within the host cell, but in the chronic stage, a durable cyst wall is synthesized, which provides protection to the parasite during transmission to a new host.\n            <jats:italic>Toxoplasma<\/jats:italic>\n            secretes proteins into the vacuole to build its replicative niche, and previous studies identified many of these proteins as phosphorylated. We investigate two secreted proteins and show that a phosphorylated region plays an important role in their regulation in acute stages. We also observed widespread phosphorylation of secreted proteins when parasites convert from acute to chronic stages, providing new insight into how the cyst wall may be dynamically regulated.\n          <\/jats:p>","DOI":"10.1128\/msphere.00792-20","type":"journal-article","created":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T14:26:30Z","timestamp":1599575190000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Phosphorylation of\n            <i>Toxoplasma gondii<\/i>\n            Secreted Proteins during Acute and Chronic Stages of Infection"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6417-928X","authenticated-orcid":false,"given":"Joanna C.","family":"Young","sequence":"first","affiliation":[{"name":"Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malgorzata","family":"Broncel","sequence":"additional","affiliation":[{"name":"Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom"},{"name":"Proteomics Science Technology Platform, The Francis Crick Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Teague","sequence":"additional","affiliation":[{"name":"Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matt R. 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