{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:56:15Z","timestamp":1791921375698,"version":"4.3.4"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI167886, DP2GM146456"],"award-info":[{"award-number":["R01AI167886, DP2GM146456"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["F30DE032598"],"award-info":[{"award-number":["F30DE032598"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2026,1,27]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      When susceptible bacterial cultures are treated with antibiotics, some cells can survive treatment without heritable resistance, giving rise to susceptible daughter cells in a phenomenon termed antibiotic persistence. Current models of fluoroquinolone (FQ) persistence in stationary-phase cultures posit that post-treatment resuscitation is dependent on double-stranded break (DSB) repair through RecA-mediated homology-directed repair. Previously, we reported that stationary-phase\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      does not depend on RecA to persist. In this work, we ask whether\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      FQ persisters from stationary-phase cultures suffer DSBs at all. We measured DSB formation in Levofloxacin (LVX)-treated cells recovering from treatment using strains expressing fluorescently labeled DSB-binding protein, Gam. We find that, surprisingly, the majority of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      LVX persisters survive treatment without apparent DSBs. Persisters that have evidence of DSBs take longer until their first division compared to persisters without DSBs. Additionally, the fates of their progenies suggest that persisters may cope with DSBs by repair or damage sequestration. These observations pave the way for mechanistic studies into\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      FQ persistence and highlight the need for single-cell tools to track FQ-induced damage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      is an opportunistic pathogen of significant clinical interest. When susceptible cultures of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      are treated with fluoroquinolone (FQ) antibiotics, some cells survive treatment and regrow in a phenomenon termed antibiotic persistence. Studies in\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      and other bacterial species suggest that FQ persisters survive by repairing DNA double-stranded breaks (DSBs) after antibiotic removal. In this study, we show that most stationary-phase\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      survive by avoiding DSBs rather than repairing them.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00793-25","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T14:00:29Z","timestamp":1765893629000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Stationary-phase\n                    <i>Pseudomonas aeruginosa<\/i>\n                    fluoroquinolone persisters mostly avoid DNA double-stranded breaks"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5014-6161","authenticated-orcid":false,"given":"Patricia J.","family":"Hare","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kzs4y22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biophysics, UConn Health","place":["Farmington, USA"]},{"id":[{"id":"https:\/\/ror.org\/02kzs4y22","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Dental Medicine, UConn Health","place":["Farmington, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliet R.","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kzs4y22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biophysics, UConn Health","place":["Farmington, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6638-2959","authenticated-orcid":true,"given":"Wendy W. K.","family":"Mok","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kzs4y22","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biophysics, UConn Health","place":["Farmington, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.3.377-392.1997"},{"key":"e_1_3_3_3_2","volume-title":"Principles of antimicrobial therapy of Pseudomonas aeruginosa infections","author":"Kanj SS","year":"2024","unstructured":"Kanj SS. 2024. Edited by KK Hall. Principles of antimicrobial therapy of Pseudomonas aeruginosa infections."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099390"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.030932-0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08506-6"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1804218115"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00731-15"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","unstructured":"Batchelder JI Mahey N Mok WWK. 2025. Post-fluoroquinolone treatment molecular events and nutrient availability modulate Staphylococcus aureus antibiotic persistence. bioRxiv:2025.06.26.661800. doi:10.1101\/2025.06.26.661800","DOI":"10.1101\/2025.06.26.661800"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03567-23"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011840"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01222"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.embor709"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1816606115"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.15252\/msb.202311672"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.23.6746-6751.2001"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2004.11.005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)74210-3"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001194"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0196-3"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.42.26424"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01617-06"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09058-4"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay3041"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00793-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00793-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T14:01:10Z","timestamp":1769522470000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00793-25"}},"subtitle":[],"editor":[{"given":"Paul D.","family":"Fey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,27]]}},"alternative-id":["10.1128\/msphere.00793-25"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00793-25","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.08.29.673110","asserted-by":"object"}]},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]},"assertion":[{"value":"2025-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00793-25"}}