{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:32:53Z","timestamp":1787016773610,"version":"build-2736575974"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01 AI169739"],"award-info":[{"award-number":["R01 AI169739"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["K12 GM088021,P20 GM121176"],"award-info":[{"award-number":["K12 GM088021,P20 GM121176"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,2,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Malaria is a highly lethal infectious disease caused by\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      parasites. These parasites are transmitted to vertebrate hosts when mosquitoes of the\n                      <jats:italic>Anopheles<\/jats:italic>\n                      genus probe for a blood meal. Sporozoites, the infectious stage of\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      , transit to the liver within hours of injection into the dermis. Vaccine efforts are hindered by the complexity of the parasite\u2019s lifecycle and the speed at which the infection is established in the liver. In an effort to enhance immunity against\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      , we produced a virus-like particle (VLP)-based vaccine displaying an epitope of TRIO, an\n                      <jats:italic>Anopheles<\/jats:italic>\n                      salivary protein that has been shown to enhance mobility and dispersal of sporozoites in the dermis. Previous work demonstrated that passive immunization with TRIO offered protection from liver infection and acted synergistically with a\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      -targeted vaccine. Immunization of mice with TRIO VLPs resulted in high-titer and long-lasting antibody responses that did not significantly drop for over 18 months post-immunization. TRIO VLPs were similarly immunogenic when combined with an anti-malaria vaccine targeting the L9 epitope of the\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      circumsporozoite protein. However, when used in a malaria challenge mouse model, TRIO VLPs only provided modest protection from infection and did not boost the protection provided by L9 VLPs.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Proteins present in the salivary glands of mosquitos have been shown to enhance the transmission efficiency of mosquito-borne pathogens, suggesting that interventions targeting the activity of these proteins could reduce transmission. Here, we looked at the efficacy of a vaccine targeting TRIO, an\n                        <jats:italic>Anopheles<\/jats:italic>\n                        mosquito salivary protein that has been reported to enhance\n                        <jats:italic>Plasmodium falciparum<\/jats:italic>\n                        malaria infection. We show that this vaccine can elicit strong anti-TRIO antibody responses, but these antibodies only result in a modest decrease in infection.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msphere.00798-24","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T09:00:36Z","timestamp":1738141236000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Virus-like particle-based vaccines targeting the\n                    <i>Anopheles<\/i>\n                    mosquito salivary protein TRIO"],"prefix":"10.1128","volume":"10","author":[{"given":"Alexandra","family":"Francian","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine","place":["Albuquerque, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yevel","family":"Flores-Garcia","sequence":"additional","affiliation":[{"name":"W. Harry Feinstone Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Powell","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine","place":["Albuquerque, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai","family":"Petrovsky","sequence":"additional","affiliation":[{"name":"Vaxine Pty Ltd.","place":["Adelaide, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1414-6196","authenticated-orcid":true,"given":"Fidel","family":"Zavala","sequence":"additional","affiliation":[{"name":"W. 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