{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:29:28Z","timestamp":1784590168390,"version":"3.55.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2024,3,26]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The type VI secretion system (T6SS) serves as a crucial molecular weapon in interbacterial competition and significantly influences the adaptability of bacteria in their ecological niche. However, the distribution and function of T6SS in clinical\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              , a common opportunistic nosocomial pathogen, have not been fully elucidated. Here, we conducted a genomic analysis of 65 clinical\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              isolates obtained from patients with varying infections. Genes encoding a T6SS cluster present in all analyzed strains of\n              <jats:italic>K. pneumoniae,<\/jats:italic>\n              and strains with identical sequence type carried structurally and numerically identical T6SS. Our study also highlights the importance of selecting conserved regions within essential T6SS genes for PCR-based identification of T6SS in bacteria. Afterward, we utilized the predominant sequence type 11 (ST11)\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              HS11286 to investigate the effect of knocking out T6SS marker genes\n              <jats:italic>hcp<\/jats:italic>\n              or\n              <jats:italic>vgrG<\/jats:italic>\n              . Transcriptome analysis identified a total of 1,298 co-upregulated and 1,752 co-downregulated differentially expressed genes in both mutants. Pathway analysis showed that only \u0394\n              <jats:italic>hcp<\/jats:italic>\n              mutant exhibited alterations in transport, establishment of localization, localization, and cell processes. The absence of\n              <jats:italic>hcp<\/jats:italic>\n              or\n              <jats:italic>vgrG<\/jats:italic>\n              gene suppressed the expression of other T6SS-related genes within the locus I cluster. Additionally, interbacterial competition experiments showed that\n              <jats:italic>hcp<\/jats:italic>\n              and\n              <jats:italic>vgrG<\/jats:italic>\n              are essential for competitive ability of ST11\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              HS11286. This study furthers our understanding of the genomic characteristics of T6SS in clinical\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              and suggests the involvement of multiple genes in T6SS of strain HS11286.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Gram-negative bacteria use type VI secretion system (T6SS) to deliver effectors that interact with neighboring cells for niche advantage.\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              is an opportunistic nosocomial pathogen that often carries multiple T6SS loci, the function of which has not yet been elucidated. We performed a genomic analysis of 65 clinical\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              strains isolated from various sources, confirming that all strains contained T6SS. We then used transcriptomics to further study changes in gene expression and its effect on interbacterial competition following the knockout of key T6SS genes in sequence type 11 (ST11)\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              HS11286. Our findings revealed the distribution and genomic characteristics of T6SS in clinical\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              . This study also described the overall transcriptional changes in the predominant Chinese ST11 strain HS11286 upon deletion of crucial T6SS genes. Additionally, this work provides a reference for future research on the identification of T6SS in bacteria.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00822-23","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T14:00:28Z","timestamp":1709560828000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["A combination of genomics and transcriptomics provides insights into the distribution and differential mRNA expression of type VI secretion system in clinical\n            <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2966-9149","authenticated-orcid":false,"given":"Wanzhen","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China"},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics, Shanghai, 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