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Once host immunity wanes, cysts reactivate to cause severe, and often lethal, toxoplasmic encephalitis. There is no available therapy to eliminate cysts or to prevent their reactivation. Furthermore, how the cyst membrane and cyst wall structures develop is poorly understood. Here, we visualized and tracked the localization of\n            <jats:italic>Toxoplasma<\/jats:italic>\n            parasitophorous vacuole membrane (PVM) dense granules (GRA) proteins during cyst development\n            <jats:italic>in vitro.<\/jats:italic>\n            PVM-localized GRA5 and GRA7 were found at the cyst membrane and cyst wall region throughout cyst development, suggesting that the PVM remains intact and develops into the cyst membrane. In addition, our results show that genetic deletion of PVM GRAs reduced the rate of accumulation of cyst wall cargo at the cyst periphery and suggest that PVM-localized GRAs mediate the development and maturation of the cyst wall and cyst membrane.\n          <\/jats:p>","DOI":"10.1128\/msphere.00851-19","type":"journal-article","created":{"date-parts":[[2020,1,14]],"date-time":"2020-01-14T13:14:50Z","timestamp":1579007690000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Toxoplasma gondii Parasitophorous Vacuole Membrane-Associated Dense Granule Proteins Regulate Maturation of the Cyst Wall"],"prefix":"10.1128","volume":"5","author":[{"given":"Rebekah B.","family":"Guevara","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, The Geisel School of Medicine at Dartmouth, Lebanon, New Hampshire, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara 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