{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T21:18:03Z","timestamp":1779139083915,"version":"3.51.4"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T00:00:00Z","timestamp":1604448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T00:00:00Z","timestamp":1608681600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI148774"],"award-info":[{"award-number":["AI148774"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,12,23]]},"abstract":"<jats:p>\n                    Tsetse flies are the insect vectors of\n                    <jats:named-content content-type=\"genus-species\">T. brucei<\/jats:named-content>\n                    , the causative agent of African sleeping sickness\u2014a zoonotic disease that inflicts a substantial economic cost on a broad region of sub-Saharan Africa. Notably, tsetse flies can be infected with the bacterium\n                    <jats:italic>S. glossinidius<\/jats:italic>\n                    to establish an asymptomatic chronic infection. This infection can be inherited by future generations of tsetse flies, allowing\n                    <jats:italic>S. glossinidius<\/jats:italic>\n                    to spread and persist within populations. To this effect,\n                    <jats:italic>S. glossinidius<\/jats:italic>\n                    has been considered a potential expression platform to create flies which reduce\n                    <jats:named-content content-type=\"genus-species\">T. brucei<\/jats:named-content>\n                    stasis and lower overall parasite transmission to humans and animals. However, the efficient genetic manipulation of\n                    <jats:italic>S. glossinidius<\/jats:italic>\n                    has remained a technical challenge due to its complex growth requirements and uncharacterized physiology. Here, we exploit a natural mechanism of DNA transfer among bacteria and develop an efficient technique to genetically manipulate\n                    <jats:italic>S. glossinidius<\/jats:italic>\n                    for future studies in reducing trypanosome transmission.\n                  <\/jats:p>","DOI":"10.1128\/msphere.00864-20","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T09:41:10Z","timestamp":1604396470000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Conjugal DNA Transfer in Sodalis glossinidius, a Maternally Inherited Symbiont of Tsetse Flies"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5614-9515","authenticated-orcid":false,"given":"Christopher G.","family":"Kendra","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Pennsylvania State University College of Medicine, 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