{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:06:16Z","timestamp":1789513576433,"version":"build-2803163510"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["R01DE033659"],"award-info":[{"award-number":["R01DE033659"]}]},{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["R01DE031159"],"award-info":[{"award-number":["R01DE031159"]}]},{"name":"HHS | NIH | National Institute of Dental and Craniofacial Research","award":["F31DE033227"],"award-info":[{"award-number":["F31DE033227"]}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2022R1C1C2007752"],"award-info":[{"award-number":["NRF-2022R1C1C2007752"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,3,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n              is a major etiological agent of periodontal disease, and infections with this bacterium are associated with systemic pathologies, including atherosclerosis, rheumatoid arthritis, and Alzheimer\u2019s disease.\n              <jats:italic>P. gingivalis<\/jats:italic>\n              has a variety of immune evasion mechanisms and exhibits highly variable cell surface characteristics that are strain dependent, complicating the development of effective vaccines and therapeutics. Here, we show that a subset of immunoglobulin M (IgM) antibodies in antiserum raised against\n              <jats:italic>P. gingivalis<\/jats:italic>\n              strain W83 selectively recognize the outer membrane vesicles (OMVs). Pre-adsorption with a mutant strain lacking an OMV-specific lipoprotein (PG1881) that has been shown to be glycosylated significantly enhanced IgM specificity toward PG1881 and the OMVs. In addition, the IgM reactivity against the OMVs derived from a mutant lacking enzymes required for O-glycosylation was markedly reduced, indicating that the IgM targets the glycan motifs on proteins carried on OMVs. Importantly, the IgM exhibited specific recognition of OMVs from both\n              <jats:italic>P. gingivalis<\/jats:italic>\n              and\n              <jats:italic>Porphyromonas endodontalis<\/jats:italic>\n              , while showing low reactivity toward other genera belonging to the phylum Bacteroidetes. This study revealed a potential host evasion strategy and highlights the potential for utilizing O-glycans in vaccine development and OMV-targeted antibodies in therapeutic interventions to combat\n              <jats:italic>P. gingivalis<\/jats:italic>\n              infections.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              O-glycosylation of cell surface proteins by bacteria is known to play a role in various functions including colonization and immune evasion. This study highlights the identification of IgM antibodies that specifically recognize O-glycosylated proteins that are selectively carried on outer membrane vesicles (OMVs). The findings suggest a potential host evasion mechanism and open new avenues for using OMVs in vaccine development and targeting O-glycans with antibodies as a therapeutic strategy against the subgingival pathobiont\n              <jats:italic>P. gingivalis<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00907-24","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T14:01:21Z","timestamp":1740578481000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Antibodies with specificity to glycan motifs that decorate OMV cargo proteins"],"prefix":"10.1128","volume":"10","author":[{"given":"Hyun Young","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology, ADA Forsyth Institute","place":["Cambridge, USA"]},{"name":"Department of Oral Microbiology and Immunology, School of Dentistry, Dental 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StatPearls Publishing Treasure Island FL."},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00569-09"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00319-19"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiac339"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12157"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12272"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00907-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00907-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T15:22:50Z","timestamp":1742916170000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00907-24"}},"subtitle":[],"editor":[{"given":"Paul D.","family":"Fey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,25]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3,25]]}},"alternative-id":["10.1128\/msphere.00907-24"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00907-24","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,25]]},"assertion":[{"value":"2024-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00907-24"}}