{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T20:46:09Z","timestamp":1775767569149,"version":"3.50.1"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T00:00:00Z","timestamp":1604448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T00:00:00Z","timestamp":1608681600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802065"],"award-info":[{"award-number":["81802065"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,12,23]]},"abstract":"\n The emergence and spread of New Delhi metallo-\u03b2-lactamase (NDM)-producing\n Enterobacteriaceae<\/jats:italic>\n have been a serious challenge to public health, and NDM-5 shows increased resistance to carbapenems compared with other variants. NDM-5 has been identified mostly in\n E. coli<\/jats:named-content>\n but has rarely been described in\n K. pneumoniae<\/jats:named-content>\n and other\n Enterobacteriaceae<\/jats:italic>\n isolates. Here, we present the dissemination of highly similar 46-kb IncX3\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n -carrying plasmids among multiclonal\n K. pneumoniae<\/jats:named-content>\n strains in children, highlighting the horizontal gene transfer of\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n among\n K. pneumoniae<\/jats:named-content>\n strains via the IncX3 plasmid. Moreover, the IncX3\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n -carrying plasmids displayed strong stability in clinical strains when cultured in antibiotic-free medium, and the plasmid maintenance was attributed partly to conjugal transfer. Plasmid conjugation is mediated by the type IV secretion system (T4SS), and T4SS is conserved among all epidemic IncX3\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n -carrying plasmids. Therefore, combining conjugation inhibition and promotion of plasmid loss would be an effective strategy to limit the conjugation-assisted persistence of IncX3\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n -carrying plasmids.\n <\/jats:p>","DOI":"10.1128\/msphere.00917-20","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T14:41:10Z","timestamp":1604414470000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Dissemination and Stability of the\n bla<\/i>\n NDM-5<\/sub>\n -Carrying IncX3-Type Plasmid among Multiclonal Klebsiella pneumoniae Isolates"],"prefix":"10.1128","volume":"5","author":[{"given":"Wenjun","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, the Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanxiu","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu Province, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4056-3583","authenticated-orcid":false,"given":"Zhen","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00115-18"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05108-11"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02295-17"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-018-0349-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00699-19"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.12.002"},{"key":"e_1_3_2_8_2","volume-title":"Performance standards for antimicrobial susceptibility testing; 25th informational supplement","author":"CLSI","year":"2018","unstructured":"CLSI. 2018. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. CLSI document M100-S28. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp498"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00573-19"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02303-17"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28216-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13359"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00685-18"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MTBP-0016-2017"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00917-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSphere.00917-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T04:31:12Z","timestamp":1646713872000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSphere.00917-20"}},"subtitle":[],"editor":[{"given":"Ana Cristina","family":"Gales","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,12,23]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,12,23]]}},"alternative-id":["10.1128\/mSphere.00917-20"],"URL":"https:\/\/doi.org\/10.1128\/msphere.00917-20","relation":{},"ISSN":["2379-5042"],"issn-type":[{"value":"2379-5042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,23]]},"assertion":[{"value":"2020-09-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00917-20"}}