{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:50:28Z","timestamp":1792079428906,"version":"4.4.0"},"reference-count":74,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T00:00:00Z","timestamp":1603238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100013832","name":"UF | Emerging Pathogens Institute, University of Florida","doi-asserted-by":"publisher","award":["14-6"],"award-info":[{"award-number":["14-6"]}],"id":[{"id":"10.13039\/100013832","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2020,10,28]]},"abstract":"<jats:p>\n            Historical data suggest that millions of\n            <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n            parasite lineages were introduced into the Americas during the trans-Atlantic slave trade, which would suggest a paraphyletic origin of the extant isolates in the Western Hemisphere. Our analyses of whole-genome variants show that the American parasites belong to a well-supported monophyletic clade. We hypothesize that the required adaptation to American vectors created a severe bottleneck, reducing the effective introduction to a few lineages. In support of this hypothesis, we discovered genes expressed in the mosquito stages of the life cycle that have alleles with multiple, high-frequency or fixed, nonsynonymous mutations in the American populations which are rarely found in African isolates. These alleles appear to be in gene products critical for transmission through the anopheline vector. Thus, these results may inform efforts to develop novel transmission-blocking vaccines by identifying parasite proteins functionally interacting with the vector that are important for successful transmission. Further, to the best of our knowledge, these are the first whole-genome data available from Haitian\n            <jats:named-content content-type=\"genus-species\">P. falciparum<\/jats:named-content>\n            isolates. Defining the genome of these parasites provides genetic markers useful for mapping parasite populations and monitoring parasite movements\/introductions.\n          <\/jats:p>","DOI":"10.1128\/msphere.00937-20","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T13:36:22Z","timestamp":1603200982000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Genetic Markers of Adaptation of Plasmodium falciparum to Transmission by American Vectors Identified in the Genomes of Parasites from Haiti and South America"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2699-7331","authenticated-orcid":false,"given":"Massimiliano S.","family":"Tagliamonte","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA"},{"name":"Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida, USA"},{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4176-8211","authenticated-orcid":false,"given":"Charles A.","family":"Yowell","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6089-4523","authenticated-orcid":false,"given":"Maha A.","family":"Elbadry","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA"},{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0814-5530","authenticated-orcid":false,"given":"Jacques","family":"Boncy","sequence":"additional","affiliation":[{"name":"Laboratoire National de Sant\u00e9 Publique, Minist\u00e8re de la Sant\u00e9 Publique et de la Population, Port-au-Prince, Haiti"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5684-1237","authenticated-orcid":false,"given":"Christian P.","family":"Raccurt","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine and Infectious Diseases, Faculty of Medicine, University of Quisqueya, Port-au-Prince, Haiti"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3583-3291","authenticated-orcid":false,"given":"Bernard A.","family":"Okech","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA"},{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3512-2107","authenticated-orcid":false,"given":"Erica M.","family":"Goss","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, College of Agricultural and Life Sciences, University of Florida, Gainesville, Florida, USA"},{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0136-2102","authenticated-orcid":false,"given":"Marco","family":"Salemi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida, USA"},{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5772-5454","authenticated-orcid":false,"given":"John B.","family":"Dame","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA"},{"name":"Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-19554-0"},{"key":"e_1_3_2_3_2","unstructured":". 2013. 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