{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:36:27Z","timestamp":1782261387044,"version":"3.54.5"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["1R01AI146103"],"award-info":[{"award-number":["1R01AI146103"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007699","name":"University of Georgia","doi-asserted-by":"crossref","award":["Distinguished Research Professorship"],"award-info":[{"award-number":["Distinguished Research Professorship"]}],"id":[{"id":"10.13039\/100007699","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,1,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Prominent virulence traits of\n              <jats:italic>Candida albicans<\/jats:italic>\n              include its ability to produce filamentous hyphal cells and grow as a biofilm. These traits are under control of numerous transcription factors (TFs), including Brg1 and Rme1. In the reference strain SC5314, a\n              <jats:italic>brg1<\/jats:italic>\n              \u0394\/\u0394 mutant has reduced levels of biofilm\/filament production; a\n              <jats:italic>brg1<\/jats:italic>\n              \u0394\/\u0394\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 double mutant has wild-type levels of biofilm\/filament production. Here, we asked whether this suppression relationship is preserved in four additional strain backgrounds: P76067, P57055, P87, and P75010. These strains represent diverse clades and biofilm\/filament production abilities. We find that a\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 mutation restores biofilm\/filament production in a\n              <jats:italic>brg1<\/jats:italic>\n              \u0394\/\u0394 mutant of P76067, but not in\n              <jats:italic>brg1<\/jats:italic>\n              \u0394\/\u0394 mutants of P57055, P87, and P75010. We speculate that variation in activities of two functionally related TFs, Nrg1, and Ume6, may cause the strain-limited impact of the\n              <jats:italic>rme1<\/jats:italic>\n              \u0394\/\u0394 mutation.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Candida albicans<\/jats:italic>\n              is a widespread fungal pathogen. The regulatory circuitry underlying virulence traits is well studied in the reference strain background, but not in other clinical isolate backgrounds. Here, we describe a pronounced example of strain variation in the control of two prominent virulence traits, biofilm formation and filamentation.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msphere.00980-24","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T13:00:33Z","timestamp":1735650033000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Strain-limited biofilm regulation through the Brg1-Rme1 circuit in\n            <i>Candida albicans<\/i>"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7383-743X","authenticated-orcid":true,"given":"Min-Ju","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Georgia","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0868-4000","authenticated-orcid":true,"given":"Aaron P.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Georgia","place":["Athens, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00021-23"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/82_2018_144"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.157"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkad139"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019729"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.605711"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.107"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005867"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22913"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.119.400486"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.174623.114"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04787-4"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05759.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007787"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01937-22"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011109"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01521-23"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00134-23"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2024.102493"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00641-23"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00372-24"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00785-23"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00579-24:e0057924"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03498-22"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1056072"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189015"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2013.01.009"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2015.9"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029707"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.10.048"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20010-9"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01872-24"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.09.001"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00163-12"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2218163120"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00402-19"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1806002115"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110837"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2021.03.019"}],"container-title":["mSphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00980-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msphere.00980-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T14:04:39Z","timestamp":1738073079000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msphere.00980-24"}},"subtitle":[],"editor":[{"given":"Rebecca 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