{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:02:25Z","timestamp":1792022545744,"version":"4.4.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2025,3,25]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n              is an important human respiratory pathogen that causes mild-to-moderate community-acquired\n              <jats:italic>M. pneumoniae<\/jats:italic>\n              pneumonia (MPP), particularly in children. RNA editing plays a vital role in pathogen infection and host immune response, but it remains largely unknown how it could be involved in the epigenetic regulation of host response to\n              <jats:italic>M. pneumoniae<\/jats:italic>\n              infection. In the present study, we performed an epitranscriptomic analysis of adenosine to inosine (A-to-I) editing in 39 bronchoalveolar lavage fluid (BALF) samples from the severe side (SS) and the opposite side (OS) of patients with MPP. Our editome profiling identified 87 differential RNA editing (DRE) events in 50 genes, especially missense editing events that recoded C-C motif chemokine receptor-like 2 (\n              <jats:italic>CCRL2<\/jats:italic>\n              , p.K147R) and cyclin I (\n              <jats:italic>CCNI<\/jats:italic>\n              , p.R75G). The expression of adenosine deaminase acting on RNA (\n              <jats:italic>ADAR<\/jats:italic>\n              ) significantly increased on SS compared to OS and positively correlated with the average RNA editing level and individual DRE events. Meanwhile, functional enrichment analysis showed that DRE was observed in genes primarily associated with the negative regulation of innate immune response, type I interferon production, and cytokine production. Further comparison of SS between complicated MPP (CMPP) and non-complicated MPP (NCMPP) revealed RNA editing events associated with MPP complications, with a higher\n              <jats:italic>ADAR<\/jats:italic>\n              expression in CMPP than NCMPP. By identifying DRE events as biomarkers associated with MPP severity and complications, our editome profiling provides new insight into the potential role played by A-to-I RNA editing in modulating the host\u2019s immune system during\n              <jats:italic>M. pneumoniae<\/jats:italic>\n              infection.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Our research investigates how\n                <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                , a common respiratory pathogen, influences how our cells modify their genetic instructions. By studying RNA editing changes in bronchoalveolar lavage fluid from patients with\n                <jats:italic>M. pneumoniae<\/jats:italic>\n                pneumonia, we aim to investigate how\n                <jats:italic>M. pneumoniae<\/jats:italic>\n                infection alters epigenetics and contributes to the disease severity and complications. Understanding such epigenetic alterations not only sheds light on the mechanisms underlying\n                <jats:italic>M. pneumoniae<\/jats:italic>\n                infection but also holds potential implications for developing better diagnostic tools and therapies. Ultimately, this work may facilitate the design of more targeted treatments to alleviate the impact of respiratory infections caused by the pathogen. Our findings may also offer broader insights into how microbial infections reshape immune processes, highlighting the importance of RNA editing in host-pathogen interactions.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/msphere.01012-24","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T14:11:04Z","timestamp":1740492664000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["BALF editome profiling reveals A-to-I RNA editing associated with severity and complications of\n            <i>Mycoplasma pneumoniae<\/i>\n            pneumonia in children"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7255-2248","authenticated-orcid":false,"given":"Yun-Yun","family":"Jin","sequence":"first","affiliation":[{"name":"Laboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University","place":["Wuxi, China"]},{"name":"Joint Primate Research Center for Chronic Diseases, Institute of Zoology of Guangdong Academy of Science, Jiangnan University","place":["Wuxi, China"]},{"name":"MOE Medical Basic Research Innovation Center for Gut Microbiota and Chronic Diseases, Wuxi School of Medicine, Jiangnan University","place":["Wuxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine & Clinical Allergy Center, Affiliated Children\u2019s Hospital of Jiangnan University (Wuxi Children\u2019s Hospital)","place":["Wuxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Wan","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine & Clinical Allergy Center, Affiliated Children\u2019s Hospital of Jiangnan University (Wuxi Children\u2019s Hospital)","place":["Wuxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University","place":["Wuxi, China"]},{"name":"MOE Medical Basic Research Innovation Center for Gut Microbiota and Chronic Diseases, Wuxi School of Medicine, Jiangnan University","place":["Wuxi, China"]},{"name":"Department of Ophthalmology, Affiliated Hospital of Jiangnan University","place":["Wuxi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8714-2543","authenticated-orcid":true,"given":"Jian-Huan","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University","place":["Wuxi, China"]},{"name":"Joint Primate Research Center for Chronic Diseases, Institute of Zoology of Guangdong Academy of Science, Jiangnan 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