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The ability to generate molecular tools to analyze viral isolates from the current outbreak is essential for furthering our understanding of how these viruses cause congenital defects. The majority of existing viral isolates and infectious cDNA clones generated from them have undergone various numbers of passages in cell culture and\/or suckling mice, which is likely to result in the accumulation of adaptive mutations that may affect viral properties. The approach described herein allows rapid generation of new, fully functional Zika virus isolates directly from deep sequencing data from virus-infected tissues without the need for prior virus passaging and for the generation and propagation of full-length cDNA clones. The approach should be applicable to other medically important flaviviruses and perhaps other positive-strand RNA viruses.<\/jats:p>","DOI":"10.1128\/mspheredirect.00190-17","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T20:30:43Z","timestamp":1495053043000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["<i>De Novo<\/i>\n                    Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case"],"prefix":"10.1128","volume":"2","author":[{"given":"Yin Xiang","family":"Setoh","sequence":"first","affiliation":[{"name":"Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie 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