{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:34:50Z","timestamp":1787016890332,"version":"build-2736575974"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Department of Biotechnology, Ministry of Science and Technology","award":["102\/IFD\/SAN\/3321\/2014-15"],"award-info":[{"award-number":["102\/IFD\/SAN\/3321\/2014-15"]}]},{"name":"Department of Science and Technology, Ministry of Science and Technology","award":["DST-Purse-11"],"award-info":[{"award-number":["DST-Purse-11"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSphere"],"published-print":{"date-parts":[[2017,8,30]]},"abstract":"<jats:p>\n                    Aminoacyl-tRNA synthetases are housekeeping enzymes essential for protein translation, providing charged tRNAs for the proper construction of peptide chains. These enzymes provide raw materials for protein translation and also ensure fidelity of translation.\n                    <jats:italic>L. donovani<\/jats:italic>\n                    is a protozoan parasite that causes visceral leishmaniasis. It is a continuously proliferating parasite that depends heavily on efficient protein translation. Lysyl-tRNA synthetase is one of the aaRSs which charges lysine to its cognate tRNA. Two different coding sequences for lysyl-tRNA synthetases (\n                    <jats:italic>Ld<\/jats:italic>\n                    LysRS) are present in this parasite.\n                    <jats:italic>Ld<\/jats:italic>\n                    LysRS-1 is closer to apicomplexans and eukaryotes, whereas\n                    <jats:italic>Ld<\/jats:italic>\n                    LysRS-2 is closer to bacteria. Here, we have characterized\n                    <jats:italic>Ld<\/jats:italic>\n                    LysRS-1 of\n                    <jats:italic>L. donovani<\/jats:italic>\n                    .\n                    <jats:italic>Ld<\/jats:italic>\n                    LysRS-1 appears to be an essential gene as the chromosomal null mutants did not survive. The heterozygous mutants showed slower growth kinetics and exhibited attenuated virulence. This study also provides a platform to explore\n                    <jats:italic>Ld<\/jats:italic>\n                    LysRS-1 as a potential drug target.\n                  <\/jats:p>","DOI":"10.1128\/mspheredirect.00340-17","type":"journal-article","created":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T20:30:25Z","timestamp":1504125025000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Genetic Validation of\n                    <i>Leishmania donovani<\/i>\n                    Lysyl-tRNA Synthetase Shows that It Is Indispensable for Parasite Growth and Infectivity"],"prefix":"10.1128","volume":"2","author":[{"given":"Sanya","family":"Chadha","sequence":"first","affiliation":[{"name":"School of Life Sciences, Jawaharlal Nehru University, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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