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TB therapy is challenging because it requires a minimum of 6 months of treatment with multiple drugs. Protracted treatment times and the emergent spread of drug-resistant\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            necessitate the identification of novel targets for drug discovery to curb this global health threat. Essential functions, defined as those indispensable for growth and\/or survival, are potential targets for new antimicrobial drugs. In this study, we aimed to define gene essentialities of\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            on a genomewide scale to comprehensively identify potential targets for drug discovery. We utilized a combination of experimental (functional genomics) and\n            <jats:italic>in silico<\/jats:italic>\n            approaches (comparative genomics and flux balance analysis). Our functional genomics approach identified sets of genes whose essentiality was affected by nutrient availability. Comparative genomics revealed that not all essential genes were fully conserved within the\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            complex. Comparing sets of essential genes identified by functional genomics to those predicted by flux balance analysis highlighted gaps in current knowledge regarding\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            metabolic capabilities. Thus, our study identifies numerous potential antitubercular drug targets and provides a comprehensive picture of the complexity of\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            essential cellular functions.\n          <\/jats:p>","DOI":"10.1128\/msystems.00070-19","type":"journal-article","created":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T13:58:35Z","timestamp":1561384715000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":124,"title":["Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways"],"prefix":"10.1128","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0888-8564","authenticated-orcid":false,"given":"Yusuke","family":"Minato","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4434-2788","authenticated-orcid":false,"given":"Daryl M.","family":"Gohl","sequence":"additional","affiliation":[{"name":"University of Minnesota Genomics Center, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua M.","family":"Thiede","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy M.","family":"Chac\u00f3n","sequence":"additional","affiliation":[{"name":"Biotechnology Institute and Department of Ecology, Evolution and Behavior, University of Minnesota, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8445-2052","authenticated-orcid":false,"given":"William R.","family":"Harcombe","sequence":"additional","affiliation":[{"name":"Biotechnology Institute and Department of Ecology, Evolution and Behavior, University of Minnesota, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2347-616X","authenticated-orcid":false,"given":"Fumito","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan"},{"name":"The Japan Science and Technology Agency\/Japan International Cooperation Agency, Science and Technology Research Partnership for Sustainable Development (JST\/JICA, SATREPS), Tokyo, Japan"},{"name":"Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1188-4238","authenticated-orcid":false,"given":"Anthony D.","family":"Baughn","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Global tuberculosis report","author":"World Health Organization","year":"2017","unstructured":"World Health Organization. 2017. 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