{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:31:01Z","timestamp":1791959461279,"version":"4.3.4"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170071"],"award-info":[{"award-number":["32170071"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82470564 82270564"],"award-info":[{"award-number":["82470564 82270564"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2022JJ30916"],"award-info":[{"award-number":["2022JJ30916"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2022JJ40770"],"award-info":[{"award-number":["2022JJ40770"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Central South University Innovation-Driven Research Programme","award":["2023CXQD059"],"award-info":[{"award-number":["2023CXQD059"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Ulcerative colitis (UC) is characterized by mucosal barrier erosion, a process exacerbated by bacterial sialidases. We investigated the therapeutic efficacy of the sialidase inhibitor (SI) in UC. In a pilot randomized clinical trial, SI intervention significantly improved clinical symptoms and endoscopic outcomes in mild-to-moderate UC patients. This improvement correlated with an enrichment of butyrate-producing taxa and beneficial metabolic pathways. In a dextran sulfate sodium-induced colitis mouse model, SI attenuated inflammation and restored mucus layer integrity, accompanied by increased expression of\n                      <jats:italic toggle=\"yes\">Muc2<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Tff3<\/jats:italic>\n                      . Crucially, unlike broad-spectrum antibiotics, SI preserved microbial community resilience while specifically enriching beneficial mucolytic commensals,\n                      <jats:italic toggle=\"yes\">Akkermansia muciniphila<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Bacteroides acidifaciens<\/jats:italic>\n                      . These findings identify SI as a promising therapeutic strategy that targets sialidase activity to reinforce the mucosal barrier and restore gut homeostasis.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>The gut microbiota plays a pivotal role in maintaining mucosal integrity and intestinal homeostasis; however, dysbiosis-driven mucus layer degradation remains a hallmark of ulcerative colitis (UC). Current interventions like antibiotics often disrupt microbial diversity, exacerbating dysbiosis and failing to address mucosal thinning, which is a critical factor in UC progression. Developing strategies to reinforce the mucus barrier without compromising microbial balance is urgently needed, but such approaches remain underexplored. Our study demonstrates that sialidase inhibitors (SIs) uniquely preserve mucosal thickness by curbing microbial mucin degradation while selectively enriching beneficial taxa and butyrate-producing bacteria. Unlike antibiotics, SIs enhance mucosal protection without destabilizing microbial communities, offering a dual-action therapeutic strategy. This work bridges a critical knowledge gap, providing evidence for microbiota-targeted therapies that synergistically restore mucosal health and microbial ecology in UC.<\/jats:p>\n                    <\/jats:sec>\n                    <jats:sec>\n                      <jats:title>CLINICAL TRIALS<\/jats:title>\n                      <jats:p>\n                        This study was registered with the Chinese Clinial Trial Registry as\n                        <jats:related-object xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" document-id=\"ChiCTR2000028767\" document-id-type=\"clinical-trial-number\" source-id=\"ChiCTR\" source-id-type=\"registry-name\" source-type=\"clinical-trials-registry\" xlink:href=\"https:\/\/www.chictr.org.cn\/showprojEN.html?proj=47789\">ChiCTR2000028767<\/jats:related-object>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msystems.00194-26","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T14:05:11Z","timestamp":1783692311000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Sialidase inhibitor modulates gut microbiota and enhances mucosal protection in the treatment of ulcerative colitis"],"prefix":"10.1128","volume":"11","author":[{"given":"Yiming","family":"Zhao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05c1yfj14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology, Xiangya Hospital Central South University","place":["Changsha, China"]},{"id":[{"id":"https:\/\/ror.org\/00f1zfq44","id-type":"ROR","asserted-by":"publisher"}],"name":"Human Microbiome and Health Group, Department of Microbiology, Xiangya School of Basic Medical Sciences, Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05c1yfj14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology, Xiangya Hospital Central South University","place":["Changsha, China"]},{"id":[{"id":"https:\/\/ror.org\/00f1zfq44","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Nutrition, Xiangya Hospital Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00f1zfq44","id-type":"ROR","asserted-by":"publisher"}],"name":"Human Microbiome and Health Group, Department of Microbiology, Xiangya School of Basic Medical Sciences, Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Xu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05c1yfj14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology, Xiangya Hospital Central South University","place":["Changsha, China"]},{"id":[{"id":"https:\/\/ror.org\/00f1zfq44","id-type":"ROR","asserted-by":"publisher"}],"name":"Human Microbiome and Health Group, Department of Microbiology, Xiangya School of Basic Medical Sciences, Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Huang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00f1zfq44","id-type":"ROR","asserted-by":"publisher"}],"name":"Human Microbiome and Health Group, Department of Microbiology, Xiangya School of Basic Medical Sciences, Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0111-3600","authenticated-orcid":false,"given":"Shuijiao","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05c1yfj14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology, Xiangya Hospital Central South University","place":["Changsha, China"]},{"id":[{"id":"https:\/\/ror.org\/05c1yfj14","id-type":"ROR","asserted-by":"publisher"}],"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7366-3946","authenticated-orcid":true,"given":"Zheng","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00f1zfq44","id-type":"ROR","asserted-by":"publisher"}],"name":"Human Microbiome and Health Group, Department of Microbiology, Xiangya School of Basic Medical Sciences, Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1193-4277","authenticated-orcid":false,"given":"Xiaowei","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05c1yfj14","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology, Xiangya Hospital Central South University","place":["Changsha, China"]},{"id":[{"id":"https:\/\/ror.org\/05c1yfj14","id-type":"ROR","asserted-by":"publisher"}],"name":"National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)32126-2"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-052919-120048"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322260"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-022-00675-x"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjv223"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2018-317571"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-021-00424-6"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.35"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.02.013"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2023.05.026"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-151-7-200910060-00143"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12071378"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2023.100600"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-025-00575-9"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq166"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1219"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1002687"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty734"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab382"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0616-3"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.15562"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-66596-w"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052249"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01688-w"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv033"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq275"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt403"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bas003"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3988"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky418"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3176"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky448"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz908"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2015.00081"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.esp-0006-2017"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2020.1735606"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803124105"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature11550"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0924-8579(01)00422-8"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.07.002"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37533-6"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0603-3"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-021-01191-x"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-008-0210-y"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-03828-4"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/137.6.1681S"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2009.02.020"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1159\/000494777"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu17142373"},{"key":"e_1_3_5_55_2","doi-asserted-by":"publisher","DOI":"10.3390\/metabo10120502"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.000079"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.055061-0"},{"key":"e_1_3_5_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2022.05.008"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002738"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i12.1519"},{"key":"e_1_3_5_61_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.1075459"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.88"},{"key":"e_1_3_5_63_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012238"},{"key":"e_1_3_5_64_2","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v053a01"},{"key":"e_1_3_5_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2025.04.026"},{"key":"e_1_3_5_66_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.5c08068"},{"key":"e_1_3_5_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2025.109283"},{"key":"e_1_3_5_68_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.37.1.58"},{"key":"e_1_3_5_69_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20150226"}],"container-title":["mSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00194-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00194-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:02:40Z","timestamp":1787058160000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msystems.00194-26"}},"subtitle":[],"editor":[{"given":"Karoline","family":"Faust","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":68,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,18]]}},"alternative-id":["10.1128\/msystems.00194-26"],"URL":"https:\/\/doi.org\/10.1128\/msystems.00194-26","relation":{},"ISSN":["2379-5077"],"issn-type":[{"value":"2379-5077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,18]]},"assertion":[{"value":"2026-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00194-26"}}