{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:06:00Z","timestamp":1785420360732,"version":"3.56.0"},"reference-count":109,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["WIS06036"],"award-info":[{"award-number":["WIS06036"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Wisconsin Dairy Innovation Hub"},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["2023-67011-40337"],"award-info":[{"award-number":["2023-67011-40337"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Wisconsin Center for Integrated Agricultural Systems"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2025,9,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Stomoxys<\/jats:italic>\n                      flies are common blood-feeding pests on dairy farms and are suspected carriers of pathogenic bacteria due to their close association with manure and cattle hosts. While prior studies have used amplicon sequencing and culture-dependent methodologies to characterize the composition of the\n                      <jats:italic toggle=\"yes\">Stomoxys<\/jats:italic>\n                      microbiota, little is known about strain-level acquisition of mastitis-causing bacteria from manure by\n                      <jats:italic toggle=\"yes\">Stomoxys<\/jats:italic>\n                      or the functional diversity of\n                      <jats:italic toggle=\"yes\">Stomoxys<\/jats:italic>\n                      -associated taxa. In this study, we address these key knowledge gaps by using whole genome sequencing to provide the first comparative genomic analysis of\n                      <jats:italic toggle=\"yes\">Stomoxys-<\/jats:italic>\n                      derived\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Staphylococcaceae<\/jats:italic>\n                      isolates. Our results show that fly and manure isolates collected from the same farm system are phylogenetically interspersed, with subsequent pairwise genome alignments revealing near-identical strains and plasmids shared between the two sources. We further identify a phylogenetic clade of\n                      <jats:italic toggle=\"yes\">Mammaliicoccus sciuri<\/jats:italic>\n                      containing known mastitis agents associated with both flies and manure. Functional analysis reveals that this clade is highly enriched in xylose metabolism genes that are rare across other\n                      <jats:italic toggle=\"yes\">M. sciuri<\/jats:italic>\n                      lineages, suggesting potential niche differentiation within the genus. Collectively, our results provide strong evidence for the acquisition of fecal-associated bacteria by adult\n                      <jats:italic toggle=\"yes\">Stomoxys<\/jats:italic>\n                      flies, confirming the link between biting muscid flies and manure habitats. The intermixing of fly and manure isolates in clinically relevant taxonomic groups strongly suggests that flies serve as carriers of opportunistic mastitis-causing or other fecal-borne pathogens and may serve as important vehicles of pathogen dissemination across the dairy farm environment.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Bovine mastitis causes up to $32 billion dollars in losses annually in the global dairy industry. Opportunistic intramammary pathogens can be transmitted through incidental contact with bacteria in environmental reservoirs like manure. However, factors affecting the abundance, persistence, and spread of these bacteria are not well understood. Our research shows that mastitis pathogens are present in the guts of blood-feeding\n                        <jats:italic toggle=\"yes\">Stomoxys<\/jats:italic>\n                        (stable) flies, which develop in cow feces and bite cows. Genomic analysis of isolates from flies, manure, and mastitis cases reveals that strains and antimicrobial resistance genes are shared between these sources. Further analysis of fly gut isolates shows virulence factors and possible niche specialization, identifying fly-associated clades with known mastitis agents from mastitic cows. This strongly suggests that\n                        <jats:italic toggle=\"yes\">Stomoxys<\/jats:italic>\n                        flies play a role in the carriage and circulation of bovine mastitis pathogens from manure in dairy settings.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msystems.00215-25","type":"journal-article","created":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T13:00:27Z","timestamp":1754053227000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Phylogenetic intermixing reveals stable fly-mediated circulation of mastitis-associated bacteria in dairy settings"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7798-6435","authenticated-orcid":false,"given":"Andrew J.","family":"Sommer","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e4byj08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bacteriology, University of Wisconsin-Madison","place":["Madison, USA"]},{"name":"Microbiology Doctoral Training Program, University of Wisconsin-Madison","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis K.","family":"Worley","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e4byj08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bacteriology, University of Wisconsin-Madison","place":["Madison, USA"]},{"name":"Microbiology Doctoral Training Program, University of Wisconsin-Madison","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Sapountzis","sequence":"additional","affiliation":[{"name":"INRAE-UCA, UMR0454 MEDIS","place":["Clermont-Ferrand, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2701-5195","authenticated-orcid":true,"given":"Kerri L.","family":"Coon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01e4byj08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bacteriology, University of Wisconsin-Madison","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2017-13023"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2019.100086"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2017-12969"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2017.2283"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss6710a1"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2017.11.021"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00336-24"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-025-01458-3"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1984-29612007000400003"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1865-1682.2010.01127.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2013026"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.4137\/IJIS.S21647"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/76.1.103"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00590"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/aen.12282"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/saw087"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.2002.00361.x"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/13.4-5.567"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjae101"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/saw083"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2006.00608.x"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1591(93)90138-F"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.09.015"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12704"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2012.03.009"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2003023"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02190-17"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147954"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3739-x"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15721"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani12233288"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2023-24086"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016-12479"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxaf025"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_3_4_38_2","unstructured":"Seemann T. 2020 ABRicate. Github. https:\/\/github.com\/tseemann\/abricate."},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1107"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks261"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03617-13"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000192"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/534738"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604891103"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24448-3"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2021.109005"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab293"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1085"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01101-x"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005944"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-024-01610-3"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168160"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-161"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/258424"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.04.009"},{"key":"e_1_3_4_57_2","unstructured":"Robins-Browne RM Holt KE Ingle DJ Hocking DM Yang J Tauschek M. 2024. Are Escherichia coli pathotypes still relevant in the era of whole-genome sequencing. Available from: http:\/\/journal.frontiersin.org\/article\/10.3389\/fcimb.2016.00141\/full"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-014-0076-9"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2015.00023"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00022-13"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01583.x"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.5994-6001.1999"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.12.037"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.16352"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-020-0696-y"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2018.11.021"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2022.01.013"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz193"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1289\/EHP5251"},{"key":"e_1_3_4_70_2","doi-asserted-by":"publisher","DOI":"10.1186\/s44149-024-00110-5"},{"key":"e_1_3_4_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2024.109099"},{"key":"e_1_3_4_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00864-09"},{"key":"e_1_3_4_73_2","unstructured":"Follador R Heinz E Wyres KL Ellington MJ Kowarik M Holt KE et al.. 2024. The diversity of Klebsiella pneumoniae surface polysaccharides. Microbial Genomics. Available from: https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.000073"},{"key":"e_1_3_4_74_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2019.2657"},{"key":"e_1_3_4_75_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1501049112"},{"key":"e_1_3_4_76_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2017.03.047"},{"key":"e_1_3_4_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2023.101675"},{"key":"e_1_3_4_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(02)00215-6"},{"key":"e_1_3_4_79_2","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlab092"},{"key":"e_1_3_4_80_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000128"},{"key":"e_1_3_4_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm008"},{"key":"e_1_3_4_82_2","doi-asserted-by":"publisher","DOI":"10.1080\/2159256X.2016.1208317"},{"key":"e_1_3_4_83_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_3_4_84_2","unstructured":"Simon A. 2010. Internet. FastQC: a quality control tool for high throughput sequence data. Available from: http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc"},{"key":"e_1_3_4_85_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"e_1_3_4_86_2","unstructured":"Seemann T. 2020. Shovill. Github. https:\/\/github.com\/tseemann\/shovill."},{"key":"e_1_3_4_87_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_3_4_88_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4163"},{"key":"e_1_3_4_89_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_4_90_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.055459-0"},{"key":"e_1_3_4_91_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-595"},{"key":"e_1_3_4_92_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00008-15"},{"key":"e_1_3_4_93_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000102"},{"key":"e_1_3_4_94_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-02090-4"},{"key":"e_1_3_4_95_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf436"},{"key":"e_1_3_4_96_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"e_1_3_4_97_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.157.2.690-693.1984"},{"key":"e_1_3_4_98_2","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01133-18"},{"key":"e_1_3_4_99_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.126"},{"key":"e_1_3_4_100_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07641-9"},{"key":"e_1_3_4_101_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2021.100802"},{"key":"e_1_3_4_102_2","unstructured":"Kassambara A factoextra MF. 2020. Internet. Extract and visualize the results of multivariate data analyses. Available from: https:\/\/CRAN.R-project.org\/package=factoextra"},{"key":"e_1_3_4_103_2","unstructured":"KoldeR. 2019. Pheatmap: Pretty Heatmaps. Kolde R. Available from: https:\/\/CRAN.R-project.org\/package=pheatmap"},{"key":"e_1_3_4_104_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_3_4_105_2","first-page":"135","volume-title":"Protein Function Prediction [Internet]","year":"2017","unstructured":"Enzyme Annotation and Metabolic Reconstruction Using KEGG, p 135\u2013145. 2017. In Protein Function Prediction [Internet]. Springer New York, New York, NY. http:\/\/link.springer.com\/10.1007\/978-1-4939-7015-5_11."},{"key":"e_1_3_4_106_2","unstructured":"Tenenbaum D. 2017. KEGGREST [Internet]. Bioconductor. https:\/\/bioconductor.org\/packages\/KEGGREST."},{"key":"e_1_3_4_107_2","unstructured":"Wickham H Chang W. 2024 Internet. Package \u2018ggplot2\u2019. create elegant data visualisations using the grammar of graphics. Available from: https:\/\/link.springer.com\/10.1007\/978-0-387-98141-3"},{"key":"e_1_3_4_108_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"e_1_3_4_109_2","doi-asserted-by":"crossref","unstructured":"Hackl T Ankenbrand MJ Adrichem B. 2024. Internet. Gggenomes: A Grammar of Graphics for Comparative Genomics. Available from: https:\/\/github.com\/thackl\/gggenomes","DOI":"10.32614\/CRAN.package.gggenomes"},{"key":"e_1_3_4_110_2","unstructured":"Schwengers O Jelonek L Dieckmann MA Beyvers S Blom J Goesmann A. 2024 Bakta: rapid and standardized annotation of bacterial genomes via alignment-free sequence identification: find out more about bakta the motivation challenges and applications here. Microbial Genomics. Available from: https:\/\/www.microbiologyresearch.org\/content\/journal\/mgen\/10.1099\/mgen.0.000685"}],"container-title":["mSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00215-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00215-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:01:38Z","timestamp":1758632498000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msystems.00215-25"}},"subtitle":[],"editor":[{"given":"Ryan J.","family":"Newton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,23]]},"references-count":109,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,23]]}},"alternative-id":["10.1128\/msystems.00215-25"],"URL":"https:\/\/doi.org\/10.1128\/msystems.00215-25","relation":{},"ISSN":["2379-5077"],"issn-type":[{"value":"2379-5077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,23]]},"assertion":[{"value":"2025-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00215-25"}}