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With the assistance of Hfq, small RNAs in bacteria play important roles in regulating the stability and translation of mRNAs by base pairing. In this study, we want to elucidate the Hfq-assisted sRNA-mRNA regulation in\n                    <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n                    . A global map of Hfq interaction sites in\n                    <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n                    was obtained by sequencing cDNAs converted from the Hfq-bound RNA fragments using UV cross-linking coupled immunoprecipitation technology. We demonstrate that Hfq could bind to hundreds of sRNAs and the majority of mRNAs in\n                    <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n                    . The enriched binding motifs in sRNAs and mRNAs are complementary to each other, suggesting a general base-pairing mechanism for sRNA-mRNA interaction. The Hfq-bound sRNA and mRNA regions were both destabilized. The results suggest that Hfq binding facilitates sRNA-mRNA base pairing and coordinates their degradation, which might enable Hfq to surveil the homeostasis of most mRNAs in bacteria.\n                  <\/jats:p>","DOI":"10.1128\/msystems.00245-19","type":"journal-article","created":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T09:03:51Z","timestamp":1563267831000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Hfq Globally Binds and Destabilizes sRNAs and mRNAs in Yersinia pestis"],"prefix":"10.1128","volume":"4","author":[{"given":"Yanping","family":"Han","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 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