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The factors that contribute to the emergence of HMV subpopulations remain unclear. In this study, eight\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              strains were recovered from an inpatient who had been hospitalized for 20 days. Three of the isolates exhibited a non-HMV phenotype, which was concomitant with higher biofilm formation than the other five HMV isolates. All eight isolates were highly susceptible to serum killing, albeit HMV strains were remarkably more infective than non-HMV counterparts in a mouse model of infection. Whole genome sequencing (WGS) showed that the eight isolates belonged to the K57-ST412 lineage. Average nucleotide identity (FastANIb) analysis indicated that eight isolates share 99.96% to 99.99% similarity and were confirmed to be the same clone. Through comparative genomics analysis, 12 non-synonymous mutations were found among these isolates, eight of which in the non-HMV variants, including\n              <jats:italic>rmpA<\/jats:italic>\n              (c.285delG) and\n              <jats:italic>wbaP<\/jats:italic>\n              (c.1305T &gt; A), which are assumed to be associated with the non-HMV phenotype. Mutations in\n              <jats:italic>manB<\/jats:italic>\n              (c.1318G &gt; A),\n              <jats:italic>dmsB<\/jats:italic>\n              (c.577C &gt; T) and\n              <jats:italic>tkt<\/jats:italic>\n              (c.1928C &gt; A) occurred in HMV isolates only. RNA-Seq revealed transcripts of genes involved in energy metabolism, carbohydrate metabolism and membrane transport, including\n              <jats:italic>cysP<\/jats:italic>\n              ,\n              <jats:italic>cydA<\/jats:italic>\n              ,\n              <jats:italic>narK<\/jats:italic>\n              ,\n              <jats:italic>tktA<\/jats:italic>\n              ,\n              <jats:italic>pduQ<\/jats:italic>\n              ,\n              <jats:italic>aceB<\/jats:italic>\n              ,\n              <jats:italic>metN,<\/jats:italic>\n              and\n              <jats:italic>lsrA<\/jats:italic>\n              , to be significantly dysregulated in the non-HMV strains, suggesting a contribution to HMV phenotype development. This study suggests that co-occurrence of HMV and non-HMV phenotypes in the same clonal population may be mediated by mutational mechanisms as well as by certain genes involved in membrane transport and central metabolism.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              with a hypermucoviscosity (HMV) phenotype is a community-acquired pathogen that is associated with increased invasiveness and pathogenicity, and underlying diseases are the most common comorbid risk factors inducing metastatic complications. HMV was earlier attributed to the overproduction of capsular polysaccharide, and more data point to the possibility of several causes contributing to this bacterial phenotype. Here, we describe a unique event in which the same clonal population showed both HMV and non-HMV characteristics. Studies have demonstrated that this process is influenced by mutational processes and genes related to transport and central metabolism. These findings provide fresh insight into the mechanisms behind co-occurrence of HMV and non-HMV phenotypes in monoclonal populations as well as potentially being critical in developing strategies to control the further spread of HMV\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msystems.00262-24","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T13:01:36Z","timestamp":1718974896000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Co-occurrence of ST412\n            <i>Klebsiella pneumoniae<\/i>\n            isolates with hypermucoviscous and non-mucoviscous phenotypes in a short-term hospitalized patient"],"prefix":"10.1128","volume":"9","author":[{"given":"Qinghua","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Pathogenic Biology, School of Basic Medical, Southwest Medical University, Luzhou, China"},{"name":"Department of Laboratory Medicine, Yilong County People\u2019s Hospital, Nanchong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology, School of Basic Medical, Southwest Medical University, Luzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology, School of Basic Medical, Southwest Medical University, Luzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology, School of Basic Medical, Southwest Medical University, Luzhou, 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