{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:10:01Z","timestamp":1773007801436,"version":"3.50.1"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100009880","name":"Regione Lazio","doi-asserted-by":"publisher","award":["RU-2014-1020"],"award-info":[{"award-number":["RU-2014-1020"]}],"id":[{"id":"10.13039\/501100009880","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009880","name":"Regione Lazio","doi-asserted-by":"publisher","award":["RU-2014-1020"],"award-info":[{"award-number":["RU-2014-1020"]}],"id":[{"id":"10.13039\/501100009880","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2019,4,30]]},"abstract":"<jats:p>\n            Botulinum neurotoxins, the causative agents of the potentially fatal disease of botulism, are produced by certain\n            <jats:italic>Clostridium<\/jats:italic>\n            strains during vegetative growth, usually in anaerobic environments. Our findings indicate that, contrary to current understanding, the growth of neurotoxigenic\n            <jats:italic>C. butyricum<\/jats:italic>\n            strains and botulinum neurotoxin type E production can continue upon transfer from anaerobic to aerated conditions and that adaptation of strains to oxygenated environments requires global changes in proteomic and metabolic profiles. We hypothesize that aerotolerance might constitute an unappreciated factor conferring physiological advantages on some botulinum toxin-producing clostridial strains, allowing them to adapt to otherwise restrictive environments.\n          <\/jats:p>","DOI":"10.1128\/msystems.00299-18","type":"journal-article","created":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T15:06:26Z","timestamp":1556550386000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Type E Botulinum Neurotoxin-Producing Clostridium butyricum Strains Are Aerotolerant during Vegetative Growth"],"prefix":"10.1128","volume":"4","author":[{"given":"Serena","family":"Camerini","sequence":"first","affiliation":[{"name":"Core Facilities, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Marcocci","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences Rossi Fanelli, Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Picarazzi","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Egidio","family":"Iorio","sequence":"additional","affiliation":[{"name":"Core Facilities, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Ruspantini","sequence":"additional","affiliation":[{"name":"Core Facilities, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Pietrangeli","sequence":"additional","affiliation":[{"name":"Department of Biochemical Sciences Rossi Fanelli, Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Crescenzi","sequence":"additional","affiliation":[{"name":"Core Facilities, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1520-4826","authenticated-orcid":false,"given":"Giovanna","family":"Franciosa","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanit\u00e0, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"738","volume-title":"Bergey\u2019s manual of systematic bacteriology","author":"Rainey FA","year":"2009","unstructured":"Rainey FA, Hollen BJ, Small AGI. 2009. Clostridium Prazmowski 1880, 23, AL, p 738\u2013828. In De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB (ed), Bergey\u2019s manual of systematic bacteriology, 2nd ed, vol 3. Springer, New York, NY.","edition":"2"},{"key":"e_1_3_2_3_2","first-page":"635","article-title":"Aerotolerance of strictly anaerobic microorganisms and factors of defense against oxidative stress: a review","volume":"43","author":"Brioukhanov AL","year":"2007","unstructured":"Brioukhanov AL, Netrusov AI. 2007. Aerotolerance of strictly anaerobic microorganisms and factors of defense against oxidative stress: a review. Prikl Biokhim Mikrobiol 43:635\u2013652. (In Russian.)","journal-title":"Prikl Biokhim Mikrobiol"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06213.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0922-338X(99)89006-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8442-8450.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06192.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-004-0659-3"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4236\/aim.2014.44028"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac9990"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02491-15"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2007.03.002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.10.4734-4741.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01698"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/anae.2000.0346"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.9.2333-2343.2002"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00351-09"},{"key":"e_1_3_2_18_2","first-page":"3","volume-title":"Clostridium botulinum","author":"Hatheway CL","year":"1992","unstructured":"Hatheway CL. 1992. Clostridium botulinum and other clostridia that produce botulinum neurotoxin, p 3\u201320. In Hauschild AHW, Dodds KL (ed), Clostridium botulinum. Marcel Dekker, New York, NY."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000000597"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2017.22.24.30550"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2160-2162.1997"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2007.08.013"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01351-15"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268814002672"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.55.1.165"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28561-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071324"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-9"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2005.02727.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.TBS-0010-2012"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.684969"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00217"},{"key":"e_1_3_2_33_2","first-page":"3","article-title":"Oxidative stress in bacteria and protein damage by reactive oxygen species","volume":"3","author":"Cabiscol E","year":"2000","unstructured":"Cabiscol E, Tamarit J, Ros J. 2000. Oxidative stress in bacteria and protein damage by reactive oxygen species. Int Microbiol 3:3\u20138.","journal-title":"Int Microbiol"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13892"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.116.012658"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2017.10.012"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1070"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2010.06.004"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00121-16"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-009-9491-y"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00751"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/154.2.207"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-002-0816-z"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ468"},{"key":"e_1_3_2_45_2","volume-title":"Botulism in the United States, 1899\u20131996, handbook for epidemiologists, clinicians and laboratory workers.","author":"Centers for Disease Control and Prevention","year":"1998","unstructured":"Centers for Disease Control and Prevention. 1998. Botulism in the United States, 1899\u20131996, handbook for epidemiologists, clinicians and laboratory workers. Centers for Disease Control and Prevention, Atlanta, GA."},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950914h"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1172"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-203"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700240-MCP200"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-3833"}],"container-title":["mSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSystems.00299-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSystems.00299-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T05:14:23Z","timestamp":1646716463000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSystems.00299-18"}},"subtitle":[],"editor":[{"given":"David","family":"Fenyo","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,4,30]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,4,30]]}},"alternative-id":["10.1128\/mSystems.00299-18"],"URL":"https:\/\/doi.org\/10.1128\/msystems.00299-18","relation":{},"ISSN":["2379-5077"],"issn-type":[{"value":"2379-5077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,30]]},"assertion":[{"value":"2018-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00299-18"}}