{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:59:27Z","timestamp":1790085567817,"version":"4.0.1"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014131","name":"McGill University Health Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014131","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2026,9,22]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Antibiotics are known to trigger prophage induction in controlled laboratory settings, but it remains unclear whether this also occurs within microbiomes in nature. Current methods investigating the link between antibiotics and prophage induction within the human gut rely on\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      culturing of human gut bacterial isolates. Using a metagenomic approach, we aimed to measure prophage induction, and whether it is associated with antibiotic exposure. In two independent human cohorts, we compared prophage to bacterial host read depth ratios (P:H) across known or measured antibiotic exposures. We found that induction is not broadly associated with antibiotic exposures at the level of the overall microbiome, but that ciprofloxacin increases P:H ratios in certain bacterial species. We documented heterogeneous trajectories of P:H ratios over the course of antibiotic exposure, sometimes increasing and remaining high, or returning to baseline. This study complements experimental models by providing\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      evidence of induction in the human gut.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Bacteriophages are viruses that infect a bacterial host. The lytic and lysogenic cycles are the two classic outcomes of phage infection. In the lytic cycle, the phage immediately replicates and lyses its host cell to release new viral particles. In the lysogenic cycle, the phage, now called a prophage, integrates its genome into that of its host without killing it. Prophages can switch to the lytic cycle in a process called induction, in which the viral genome is replicated, the host cell is lysed, and viral particles are released. The most immediate consequence of induction is host cell death, which can impact bacterial populations and communities. Since prophages are mobile genetic elements that can move between bacteria, they are also an important vehicle for horizontal gene transfer. While induction has been well studied\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        , whether and how induction occurs within the complex microbial ecosystem in humans is less well characterized. Understanding prophage induction\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        is therefore critical in corroborating\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        observations.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msystems.00303-26","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:06:47Z","timestamp":1787576807000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Species-specific prophage induction by ciprofloxacin in human gut metagenomes"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1576-7136","authenticated-orcid":true,"given":"Ben","family":"Sakdinan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology & Immunology, McGill University","place":["Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshul","family":"Sinha","sequence":"additional","affiliation":[{"name":"McGill Centre for Microbiome Research","place":["Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8928-9888","authenticated-orcid":true,"given":"Firdausi","family":"Qadri","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04vsvr128","id-type":"ROR","asserted-by":"publisher"}],"name":"Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh","place":["Dhaka, Bangladesh"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraful I.","family":"Khan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04vsvr128","id-type":"ROR","asserted-by":"publisher"}],"name":"Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh","place":["Dhaka, Bangladesh"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2649-2624","authenticated-orcid":true,"given":"Eric J.","family":"Nelson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02y3ad647","id-type":"ROR","asserted-by":"publisher"}],"name":"Emerging Pathogens Institute, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6819-8699","authenticated-orcid":true,"given":"B. Jesse","family":"Shapiro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology & Immunology, McGill University","place":["Montr\u00e9al, Canada"]},{"name":"McGill Centre for Microbiome Research","place":["Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007494"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14983"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13030455"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.1.171-177.2006"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01874-23"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","unstructured":"Bucher MJ Puente CP Sehdev N Czy\u017c DM. 2024. Sub-therapeutic concentrations of antibiotics induce prophage-driven superinfection exclusion and fitness cost in Pseudomonas aeruginosa. bioRxiv. doi:10.1101\/2024.11.20.624585","DOI":"10.1101\/2024.11.20.624585"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00084-22"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.adj3166"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2024.03.025"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-08781-x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","unstructured":"McKerral JC Papudeshi B Inglis LK Roach MJ Decewicz P McNair K Luque A Dinsdale EA Edwards RA. 2023. The promise and pitfalls of prophages. bioRxiv:2023.04.20.537752. doi:10.1101\/2023.04.20.537752","DOI":"10.1101\/2023.04.20.537752"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2025.03.073"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09786-2"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.12.7499-7506.2003"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.4c00041"}],"container-title":["mSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00303-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00303-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:01:26Z","timestamp":1790082086000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msystems.00303-26"}},"subtitle":[],"editor":[{"given":"Alejandro","family":"Reyes Munoz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":15,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,22]]}},"alternative-id":["10.1128\/msystems.00303-26"],"URL":"https:\/\/doi.org\/10.1128\/msystems.00303-26","relation":{},"ISSN":["2379-5077"],"issn-type":[{"value":"2379-5077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,22]]},"assertion":[{"value":"2026-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00303-26"}}