{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:41:30Z","timestamp":1785436890259,"version":"3.56.0"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22127805, 22206008"],"award-info":[{"award-number":["22127805, 22206008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Outstanding Young Scientist Program","award":["BJJWZYJH01201910005017"],"award-info":[{"award-number":["BJJWZYJH01201910005017"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760783"],"award-info":[{"award-number":["81760783"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2025,8,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Viral protein U (Vpu), an accessory protein of HIV-1, functions by antagonizing or hijacking various host factors to allow the virus to evade host immune surveillance and facilitate viral release. Nevertheless, there is limited understanding regarding the impact of Vpu on the biogenesis of HIV-1 RNAs. In this study, we utilized ascorbate peroxidase 2 (APEX2)-based proximity labeling techniques in combination with mass spectrometry and immunoprecipitation-mass spectrometry (IP-MS) to characterize interactions between HIV-1 Vpu and host proteins. We identified nine cellular targets of Vpu. Among these targets, our research demonstrated the interaction between Vpu and RNA-binding motif protein 10 (RBM10), which results in the degradation of RBM10 through the ubiquitin-proteasome pathway. The expression of RBM10 exerted an inhibitory effect on virus replication by binding to viral RNA and reducing the levels of incompletely spliced HIV-1 transcripts. Additionally, it promoted the transcription of various antiviral genes. The findings elucidate the role of HIV-1 Vpu in RNA replication and identify RBM10 as a novel regulator of HIV-1 transcription.<\/jats:p>\n                    <jats:p\/>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>A comprehensive analysis utilizing APEX2-MS and IP-MS techniques identified a total of 24 cellular targets of Vpu, three of which have been documented as restriction factors. Vpu-interacting proteins were found to be significantly enriched in pathways related to cell adhesion, RNA transport, and the spliceosome. The identification of RBM10 as a novel regulator of HIV-1 replication and infectivity, and RBM10 regulated transcription of both viral and host RNA transcripts. Vpu interacted with RBM10 and promoted its degradation through the ubiquitin-proteasome pathway.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msystems.00403-25","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T13:00:45Z","timestamp":1753966845000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["HIV-1 Vpu interacts with RBM10 to promote HIV-1 infection"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1923-4013","authenticated-orcid":false,"given":"Boye","family":"Li","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/05gfwht30","id-type":"ROR","asserted-by":"publisher"}],"name":"Civil Aviation Medicine Center, Civil Aviation Administration of China","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanzhe","family":"Hao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b1sh213","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianbin","family":"Meng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cve4549","id-type":"ROR","asserted-by":"publisher"}],"name":"MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoming","family":"Wang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of Geriatric Medicine, Chinese Academy of Medical Science, Beijing Hospital\/National Center of Gerontology of National Health Commission","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyan","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05gfwht30","id-type":"ROR","asserted-by":"publisher"}],"name":"Civil Aviation Medicine Center, Civil Aviation Administration of China","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runqing","family":"Jia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishu","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinning","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyang","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenmei","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2208-2081","authenticated-orcid":false,"given":"Xiaoguang","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04b1sh213","id-type":"ROR","asserted-by":"publisher"}],"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1735-0077","authenticated-orcid":false,"given":"Xiayan","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1196-879X","authenticated-orcid":true,"given":"Qin","family":"Hu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Chemistry and Life Science, Beijing University of Technology","place":["Beijing, China"]},{"id":[{"id":"https:\/\/ror.org\/037b1pp87","id-type":"ROR","asserted-by":"publisher"}],"name":"Beijing International Science and Technology Cooperation Base of Antivirus Drug, Beijing University of Technology","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"WHO. 2024. HIV Statistics Globally and by WHO Region on World Health Organization. Available from: https:\/\/cdn.who.int\/media\/docs\/default-source\/hq-hiv-hepatitis-and-stis-library\/j0482-who-ias-hiv-statistics_aw-1_final_ys.pdf?sfvrsn=61d39578_3"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2013.08.004"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00242-09"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007257"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06553"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13081466"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.08.039"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000574"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.003343"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00036-11"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-11-15"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.04.023"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.10.008"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-09-243667"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02442-16"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05788-11"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01920-21"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01999-19"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0753-6"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.09.003"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","unstructured":"Liu Y Fu Y Wang Q Li M Zhou Z Dabbagh D Fu C Zhang H Li S Zhang T Gong J Kong X Zhai W Su J Sun J Zhang Y Yu XF Shao Z Zhou F Wu Y Tan X. 2019. Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor 4:813\u2013825. doi:10.1038\/s41564-019-0372-2","DOI":"10.1038\/s41564-019-0372-2"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009409"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx508"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/cge.13901"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa340"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.018"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2011.09.007"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.11.022"},{"key":"e_1_3_4_30_2","first-page":"151","article-title":"Sampling variation of the fifty percent end-point, determined by the Reed-Muench (Behrens) method","volume":"22","author":"PIZZI M","year":"1950","unstructured":"PIZZI M. 1950. Sampling variation of the fifty percent end-point, determined by the Reed-Muench (Behrens) method. Hum Biol 22:151\u2013190.","journal-title":"Hum Biol"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01801-08"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2375"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.065"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(00)80056-8"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03293-22"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000450"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01183-23"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.07.032"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.06.003"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.9b00318"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells10040821"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2241"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01795-10"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813223106"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.2174\/157016212800792513"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2002.6733"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1996.0022"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab490"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12867-017-0096-x"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.08.077"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01101.x"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01800-08"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2020.113599"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2021.145463"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.10.045"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2217451120"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep40488"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2016.1144004"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14050903"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065x.1999.tb01282.x"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030104"},{"key":"e_1_3_4_63_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2023.2196847"},{"key":"e_1_3_4_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00713-17"},{"key":"e_1_3_4_65_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000273"},{"key":"e_1_3_4_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclinepi.2022.02.005"},{"key":"e_1_3_4_67_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003241"},{"key":"e_1_3_4_68_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-8-47"},{"key":"e_1_3_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-7799(03)00118-5"}],"container-title":["mSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00403-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00403-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T13:02:14Z","timestamp":1755608534000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msystems.00403-25"}},"subtitle":[],"editor":[{"given":"Aleksandra","family":"Nita-Lazar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,8,19]]},"references-count":68,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,19]]}},"alternative-id":["10.1128\/msystems.00403-25"],"URL":"https:\/\/doi.org\/10.1128\/msystems.00403-25","relation":{},"ISSN":["2379-5077"],"issn-type":[{"value":"2379-5077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,19]]},"assertion":[{"value":"2025-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00403-25"}}