{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T07:10:33Z","timestamp":1783753833512,"version":"3.55.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MOST | National Key Research and Development Program of China","award":["2021YFC2302002"],"award-info":[{"award-number":["2021YFC2302002"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2023,10,26]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antibiotic resistance has become a worldwide public health and biosafety problem. The clinical efficacy of colistin was jeopardized by the report of the first mobile colistin resistance (mcr) gene, and subsequently, the mcr-gene family of mcr-1 to mcr-10 has been identified.\n            <jats:italic>Cronobacter<\/jats:italic>\n            is an emerging foodborne opportunistic pathogen, which can cause neonatal meningitis, bacteremia, and necrotizing enterocolitis (NEC) by contaminating food. However, the entire picture of foodborne\n            <jats:italic>Cronobacter<\/jats:italic>\n            carriage of the mcr genes is not known. Here, we investigated the mcr genes of\n            <jats:italic>Cronobacter<\/jats:italic>\n            isolates (\n            <jats:italic>N<\/jats:italic>\n            = 877) by whole-genome sequencing. We found 133 of 877 previously undescribed\n            <jats:italic>Cronobacter<\/jats:italic>\n            isolates carrying mcr genes. Further genomic analysis revealed that these mcr genes mainly belonged to the mcr-9 and mcr-10. Genomic analysis of the flanking structures of mcr genes revealed that two core flanking structures were prevalent in foodborne\n            <jats:italic>Cronobacter<\/jats:italic>\n            isolates, and the flanking structure carrying IS1R was found for the first time in this study. These findings suggest that foodborne\n            <jats:italic>Cronobacter<\/jats:italic>\n            isolates carrying the mcr genes already pose a threat to human health.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Cronobacter<\/jats:italic>\n              is an emerging foodborne opportunistic pathogen, which can cause neonatal meningitis, bacteremia, and NEC by contaminating food. However, the entire picture of foodborne\n              <jats:italic>Cronobacter<\/jats:italic>\n              carriage of the mcr genes is not known. Here, we investigated the mcr genes of\n              <jats:italic>Cronobacter<\/jats:italic>\n              isolates by whole-genome sequencing and found 133 previously undescribed\n              <jats:italic>Cronobacter<\/jats:italic>\n              isolates carrying mcr genes. Further genomic analysis revealed that these mcr genes mainly belonged to the mcr-9 and mcr-10. Genomic analysis of the flanking structures of mcr genes revealed that two core flanking structures were prevalent in foodborne\n              <jats:italic>Cronobacter<\/jats:italic>\n              isolates, and the flanking structure carrying IS1R was found for the first time in this study.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msystems.00450-23","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T13:38:53Z","timestamp":1694439533000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Large-scale genomic survey and characterization of mcr genes carried by foodborne\n            <i>Cronobacter<\/i>\n            isolates"],"prefix":"10.1128","volume":"8","author":[{"given":"Qianhui","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Engineering Medicine, School of Engineering Medicine, Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Interdisciplinary Innovation Institute of Medicine and Engineering, Beihang University , Beijing, Hebei, China"},{"name":"State Key Laboratory of Microbial Resources, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"},{"name":"Institute of Microbiology, University of Chinese Academy of Sciences , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinrui","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Engineering Medicine, School of Engineering Medicine, Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Interdisciplinary Innovation Institute of Medicine and Engineering, Beihang University , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heyuan","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"},{"name":"Microbial Resource and Big Data Center, Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"},{"name":"Microbial Resource and Big Data Center, Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"},{"name":"Institute of Microbiology, University of Chinese Academy of Sciences , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songnian","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"},{"name":"Institute of Microbiology, University of Chinese Academy of Sciences , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linhuan","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"},{"name":"Microbial Resource and Big Data Center, Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijian","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Infectious Disease Prevention and Control, State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, Hebei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7111-7770","authenticated-orcid":false,"given":"Zilong","family":"He","sequence":"additional","affiliation":[{"name":"School of Engineering Medicine, School of Engineering Medicine, 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