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Dual RNAseq analysis of the cocultures also confirmed that the transcriptome of\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            was distinct during coinfection compared to single-infection scenarios where processes related to metabolism were significantly perturbed. Further,\n            <jats:italic>in silico<\/jats:italic>\n            gene-knockout simulations uncovered discrepancies in gene essentiality for\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            growth between single infections and coinfections. Integrative model-guided analysis thus identified druggable targets that would be critical for\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            growth in both single infections and coinfections; thus, designing inhibitors against those targets would provide a broader spectrum of coverage against cholera infections.\n          <\/jats:p>","DOI":"10.1128\/msystems.00491-20","type":"journal-article","created":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T13:23:30Z","timestamp":1599485010000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Integrated Metabolic Modeling, Culturing, and Transcriptomics Explain Enhanced Virulence of Vibrio cholerae during Coinfection with Enterotoxigenic Escherichia coli"],"prefix":"10.1128","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3114-1100","authenticated-orcid":false,"given":"Alyaa 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